The Vitamin D Receptor Redistribution Assay monitors the cellular translocation of GFP-tagged VDR in response to drug compounds or other stimuli and allows easy acquisition of multiple readouts from the same cell in a single assay run.
Product Detail
Vitamin D Receptor Redistribution Assay Features
Designed to assay compounds for their ability to modulate nuclear translocation of the Vitamin D Receptor
Coupled to EGFP for easy monitoring of the cellular translocation event
Robust cell-based assay for use in high content analysis and fluorescence microscope applications
Vitamin D Receptor Redistribution Assay Highlights
Biologically relevant data: Compounds tested in a cellular environment
Validated: Functionally tested cells provided with an optimized assay protocol
Easy to use: Just plate cells, add compounds, and image
The vitamin D receptor (VDR) is a member of the steroid receptor (nuclear hormone receptor) family and mediates gene expression in a vitamin D/calcitriol dependent fashion. Calcitriol, the active form of vitamin D, regulates various aspects of calcium metabolism and bone homeostasis. In addition, vitamin D and analogs thereof also possess potent differentiative, anti-proliferative, and immunomodulatory activities, and is currently used clinically to treat psoriasis. VDR functions as a sequence-specific transcription factor as a hetero-dimer with the Retinoid X Receptor (RXR). In the absence of ligand, VDR complexes with co-repressor proteins to repress target genes. Binding of ligand to the receptor induces a conformational shift in the receptor and allows the exchange of co-repressors with co-activators and activation of target genes.
Figure 1. Nuclear translocation of EGFP-VDR. Cells were treated with 300 nM Vitamin D3 for 30 min (right panel) or untreated (DMSO control, left panel). Arrows indicate the nuclear localization of EGFP-VDR detected by the image analysis algorithm.
Figure 2. Vitamin D3 concentration response in the Vitamin D Receptor assay.Concentration response was measured in 9 point half log dilution series (n=16). The EC50 is approximately 3 nM. Cells were treated with Vitamin D3 for 30 min.. Cells were then fixed and nuclear translocation was measured using the Cellomics ArrayScan VTI Reader and the Redistribution V3 BioApplication. % activity was calculated relative to the positive (300 nM Vitamin D3) and negative control (0.25% DMSO).
1 Articles Total 7,000.00 USD 
