Thermo Scientific HyClone HyPure Molecular Biology Grade Water is processed and tested for use in critical applications.
Product Detail
In order to work with nucleotides such as RNA and DNA and to prevent subsequent degradation due to enzymatic activity, it is necessary to use reagents, including water, that are devoid of all nucleases. Nucleases are naturally occurring enzymes whose role is specifically to cleave nucleotide polymeric molecules such as DNA or RNA at various points. Theoretically, just one molecule of a nuclease could systematically facilitate the destruction of an entire nucleotide sample over time. HyClone Molecular Biology Grade Water is purified, tested and certified to have no detectable contaminating activity in DNase, RNase, and Protease assays. It is very low in endotoxin and contains no DEPC or any other carcinogenic chemicals. Our purification process includes continuous deionization, reverse osmosis, ultra-violet light treatment, multi-effect distillation, and 0.1µm filtration. Applications include:
Buffer preparation DNA/RNA purification DNA sequencing and libraries Gene mapping Recombinant DNA and cloning PCR analysis Northern Blot, Southern Blot, and other assays
Nuclease-free water is certified to have no detectable contaminating activity in DNase, RNase, and Protease assays
Suitable for DNA/RNA purification, DNA sequencing and libraries, Gene mapping, Recombinant DNA and cloning, Northern Blot, Southern Blot
Maximum allowable limit of endotoxin is 0.05EU/mL
Purification process includes continuous deionization, reverse osmosis, ultraviolet light treatment, distillation, and 0.1µm filtration
Compliance: Manufactured under cGMP and ISO 9001:2000 guidelines.