The Fluoroskan Ascent FL is equipped with both fluorometric and luminometric measurement technologies, providing a full coverage for fluorometric, glow and flash luminometric applications even in the same run. Flexible1- to 384- plate formats and PCR plates, up to three on-board dispensers, an incubator and an orbital shaker support a wide variety of applications. Easy-to-use Ascent Software offers a powerful tool for data handling. Compatibility with automation makes it ideal for HTS.
Product Detail
For maximum flexibility, Fluoroskan Ascent FL is equipped with both
fluorometric and luminometric measurement technologies that complement the long
list of advanced features of Fluoroskan Ascent and Luminoskan Ascent. Fluoroskan Ascent FL provides full coverage for fluorometric, glow and flash luminometric applications. Application areas range from cell biology and immunology to molecular biology and microbiology. Together with Ascent Software, Fluoroskan Ascent FL is a high performance tool for all kinds of assays in industrial and academic research and for clinical applications. Robot-friendly hardware and software have been optimized for high-volume testing.
Application areas of Fluoroskan Ascent FL
ATP detection
FRET assays
Cell adhesion
Reporter gene assays
Calcium flux assays
Immunoassays
DNA quantitation
Cytotoxicity
Bacterial quantitation
Hybridization assays
Cell proliferation
Enzymatic activity
Quantitation of PCR products
Molecular beacon assays
Phagocytosis
State-of-the-art optical system for unrivaled sensitivity
The direct illumination optics of Fluoroskan Ascent FL produce a highly focused light beam. In fluorescence measurement, this prevents crosstalk and ensures accurate readings. Two beam diameter settings, 3 mm and 1.5 mm, allow optimal readings of 1-96 and 384-well plates. Automatic gain selection eliminates the need for manual adjustment in fluorometry. It also guarantees the best possible sensitivity and dynamic range for all wavelengths. High sensitivity is one of the main benefits of fiberless optics - and a critical feature for luminometry. Fluoroskan Ascent FL can also be equipped with filters for luminometric measurements.
Flexible plate formats, from 1 to 384, enable reading in primary wells
Designed for superior flexibility, Fluoroskan Ascent FL reads any microplate format, including 1-96 and 384-well plates. The format is simple to select from a database covering the most common plate types. Customized plate formats can be set using the template editor. Automatic plate positioning is directly controlled by the reader, ensuring correct placement in the carrier, regardless of plate dimensions.
Up to three dispensers for flash and rapid kinetic applications
For easy and accurate reagent addition, up to three dispensers can be fitted on board Fluoroskan Ascent FL. Dispensing volumes can be adjusted in 1µl increments over a volume range of 1-1000 µl. Speed and precision are key features of these dispensers. The system can dispense and measure simultaneously, which is essential for flash type luminescence reactions, fluorometric Ca2+ measurements and other rapid kinetic applications. The fast read time of Fluoroskan Ascent FL, just 15 seconds for a 96-well plate, is another important advantage for kinetic applications.
On-board incubation and orbital shaking An incubator option is available for cell biology, enzyme assays and other applications requiring precise temperature control. Built-in orbital shaking, another advanced feature, speeds up reaction times and ensures effective mixing. Both the speed and amplitude of shaking can be adjusted.
Completely robot compatible Robotic integration is simple and effective with the Fluoroskan Ascent FL.
The plate carrier allows convenient access for different kinds of robotic arms. It has been designed to enable portrait gripping of the plate with the gripper of less than 60 mm and also landscape gripping with the gripper of less than 65 mm. In addition, wall edges of the carrier have been beveled to aid the plate to slide down to the carrier.
Ascent Software is easy to integrate with robotic and LIMS systems. The execution of predefined assays can be controlled remotely and the software can be set up to export and import data automatically.
1-, 6-, 12-, 24-, 48-, 96-, and 384-well plates, Terasaki and PCR plates. Can also be programmed for custom configurations. Maximum dimensions 90 mm x 134 mm x 25 mm.
Shaker :
Orbital, speed 60-1200 rpm, diameter 1-50 mm
Dispensers :
Up to 3 dispensers
Dispensing volume :
1-1000 µl in 1 µl increments
Accuracy :
±3 µl average for volumes 5 µl and above
Precision :
5-19 µl < 5%, 20-1000 µl < 2%
Dispensing speed :
25 seconds per plate (96-well plate, 5 µl/well)
Incubator temperature range :
From RT +3C to +45C when ambient temperature is 25C°
Measuring speed :
Minimum kinetic interval time is 15 seconds for a 96-well plate
FLUOROMETRIC SPECIFICATIONS :
Light source :
Quartz halogen lamp, 30 W
Detector :
Photomultiplier tube
Excitation wavelength range :
From 320 nm to 700 nm
Emission wavelength range :
From 360 nm to 670 nm (optional from 360 nm to 800 nm)
Filters :
High-quality interference filters. The excitation filter wheel can hold a maximum of eight filters, and the emission filter wheel can hold a maximum of six filters.
Filters included :
Excitation filters: 355 nm, 485 nm
Emission filters: 460 nm, 538 nm
Excitation filter selection :
320 nm, 355 nm, 390 nm, 430 nm, 440 nm, 444 nm, 485 nm, 530 nm, 544 nm, 578 nm, 584 nm. Other filters available upon request.
Emission filter selection :
405 nm, 460 nm, 485 nm, 510 nm, 518 nm, 520 nm, 527nm, 538 nm, 555 nm, 590 nm, 604 nm, 612 nm, 620 nm. Other filters available upon request.
Theoretical sensitivity :
40 amol ATP/ well using flash reaction and white 384-well plate
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