DyLight Fluorescent Labeled Phosphoramidites
 
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  DyLight Fluorescent Labeled Phosphoramidites


DyLight™ Phosphoramidites feature absorption and emission spectra suitable for a broad range of applications. They can be used in research applications without the constraints of licensing and royalty fees.
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DyLight labeled phosphoramidites are used to introduce a fluorescent tag at the 5'-position of an oligonucleotide. They are easily incorporated into the oligo using standard synthesis and deprotection procedures. Fluorescent labeled oligonucleotides are important tools in diagnostics and gene expression analysis.

DY547, DY647 & DY677
DyLight DY547, DY647 and DY677 phosphoramidites have absorbance and emission spectra comparable to Cy3, Cy5 and Cy 5.5 respectively.  DyLight labeled oligonucleotides are stable from pH 3-9 and have photostability comparable to or exceeding Oligos labeled with Cy dyes. Thermo Fisher Scientific does not require the payment of licensing fees for use of DyLight dyes in research applications.

DY780
DyLight DY780 phosphoramidite is used in fluorescent applications requiring emission in the near IR range.

FAM-6 and Fluorescein
FAM-6 and fluorescein labeled oligonucleotides are used in sequencing and PCR applications.  These phosphoramidites may be used on all automated DNA synthesizers using standard synthesis procedures. FAM6 is further purified to remove the 5-isomer and leaves ≥ 95% of the 6-isomer.  The higher purity of the single isomer is ideal in applications requiring higher sensitivity.

Quality Control
Our controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply.  Processes are strictly controlled and monitored from material procurement through final QC and product release under ISO9001 standards.  All phosphoramidites are supplied with lot-specific quality control data.

Flexible Packaging
Pack sizes to fit most commercially available synthesizers are in stock and ready for immediate delivery.  Custom and bulk packaging is also available for specific requirements.  Contact us to request samples for evaluation.

Related Products for Mild Deprotection
It is recommended to use these products with Thermo Scientific Fast Deprotection Phosphoramidites during synthesis to allow for mild deprotection protocols and minimize fluorescent label degradation.

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 DyLight Fluorescent Labeled Phosphoramidites
 Product# Price  Image  Product Name  
         
 SY633203      -  
 
 		  DyLight DY547 Phosphoramidite,
100 µmol		   SELECT  
 SY633403      -  
 
 		  DyLight DY647 Phosphoramidite,
100 µmol		   SELECT  
 SY633001      -  
 
 		  DyLight DY780 Phosphoramidite,
50 mg		   SELECT  
 27-1606-00      -  
 
 		  DyLight Fluorescein Phosphoramidite,
custom pack size		   SELECT  
 27-1606-01      -  
 
 		  DyLight Fluorescein Phosphoramidite,
100 mg		   SELECT  
 27-1606-03      -  
 
 		  DyLight Fluorescein Phosphoramidite,
100 µmol		   SELECT  
 27-1606-04      -  
 
 		  DyLight Fluorescein Phosphoramidite,
250 mg		   SELECT  
 27-1608-00      -  
 
 		  DyLight FAM-6 Phosphoramidite,
custom pack size		   SELECT  
 27-1608-03      -  
 
 		  DyLight FAM-6 Phosphoramidite,
100 µmol		   SELECT  
 27-1608-04      -  
 
 		  DyLight FAM-6 Phosphoramidite,
250 mg		   SELECT  
 SY633603      -  
 
 		  DyLight DY677 Phosphoramidite,
100 µmol		   SELECT  
 SY633604      -  
 
 		  DyLight DY677 Phosphoramidite,
250 mg		   SELECT  
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