Thermo Scientific Verso 2-step QRT-PCR Kits include both the RT and QPCR components required for highly sensitive, 2-step amplification of RNA templates. The kits combine the high performance of the Verso RT enzyme and the unique advantages of ABsolute™ Blue QPCR Master Mixes, creating an advanced system that confers superior dynamic range and enhanced sensitivity.
The Verso 2-Step QRT-PCR Kit has unparalleled sensitivity and is able to detect as little as 1pg of total RNA or mRNA. To increase sensitivity to this level, the kit includes separate vials of random hexamers and anchored oligo dT primers for primer-template optimization. The combination of these two priming strategies gives sensitivity equal to that delivered by gene specific primers and also gives greater coverage and higher yield of cDNA from RNA.
The Verso RT enzyme has high thermal-stability and can be used at temperatures up to 57°C, avoiding problems caused by secondary structure in the RNA template. The Verso enzyme mix performs the reverse transcription step much faster than other enzymes. This decreases the cDNA transcription time to 15 minutes, resulting in reduced protocol times. The QPCR step is optimized with the use of ABsolute Blue QPCR mixes. Proprietary additives give enhanced and consistent end point readings as well as low Ct values. All mixes contain Thermo-Start Taq DNA Polymerase, for increased specificity, and contain a high performance dNTP mix (with dTTP) for increased reaction efficiency and sensitivity.
Verso QRT-PCR Kits contain an inert blue dye to significantly enhance the contrast between reagent and plastic. The blue color does not effect the QPCR reaction, and offers an immediate check that a well has not been skipped or miss-aliquoted. These blue mixes are ideal for use with white reaction plates and tubes. An increase in detection efficiency is seen when using ABgene white PCR plastics for QPCR. While white plastics give superior QPCR results, they can make traditional colorless reagents difficult to see. With Verso, a simple glance ensures that the correct amount of master mix has been added.
Features
Easy-to-see mix. Verso QRT-PCR Kits contain an inert blue dye for easy sample visualization during reaction set-up.
High yield cDNA. Verso 2-Step QRT-PCR Kits contain the highly-efficient Verso RT enzyme with an optimized buffer designed to produce high yield, full length cDNA from even low copy number templates.
Flexible priming methods. Random hexamers and anchored oligo dT primers are supplied separately to allow for complete primer optimization.
Unique RT Enhancer. Verso QRT-PCR kits contain RT Enhancer, a unique enzyme that degrades any contaminating genomic DNA during the RT step. It eliminates the need for a DNase I treatment of extracted RNA, saving time and effort.
Maximum specificity. Chemically-modified Thermo-Start Taq remains completely inactive until heated to 95ºC for 15 minutes, thereby preventing non-specific amplification.
Instrument specific. Individually optimized QPCR mixes designed for all major QPCR instruments. Mixes are also available for use with probes or SYBR Green chemistry.
Reproducible results. All mixes undergo extensive quality control testing to ensure batch-to-batch consistency.
Kit Components
Verso Enzyme Mix, including RNase Inhibitor
cDNA Synthesis Buffer (5X)
Random Hexamers
High performance dNTPs
RT Enhancer
ABsolute Blue QPCR Mix (2X), including:
Thermo-Start DNA Polymerase
High performance dNTPs
MgCl2
SYBR Green I (only in SYBR Green mixes)
ROX Passive Reference Dye (in select mixes)
Separate MgCl2 vial for further MgCl2 optimization (only in SYBR Green kits)
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