Conclusion:

A simple, rapid and sensitive method for analysis of CAP in milk by LC-MS-MS has been developed and validated. The sample preparation by protein precipitation and dilution is very simple to perform and avoids the use of SPE or LLE. With the high-speed Accela LC coupled to a triple quadruple TSQ Quantum Access, each analytical run is as short as 3 min. The method can be used for the purposes of both high-throughput screening and rapid confirmatory assays.

For screening assay, the method can detect < 0.050 μg/kg CAP in milk. For confirmatory assay, the method validated according to Decision 2002/657/EC gives a CCα =0.087 μg/kg and CCβ = 0.12 μg/kg, both below the MRPL of 0.3 μg/kg.

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