High-Field Asymmetric-waveform Ion Mobility Spectrometry(FAIMS) has been implemented in the LC Data-Dependent full scan MS/MS analysis of Loperamide and its microsome metabolites.

FAIMS provides an orthogonal separation based onshape, size, charge and can be used to dramatically reduce chemical noise due, for example, to solvent clusters, dosing vehicles, or endogenous matrix.

In these studies we observe significant reduction in full scan MS chemical noise. In addition, implementation of FAIMS in this analysis resulted in a significant improvementin the XIC peak area S/N ratios for all mass-to-charge ratios examined.

Most notably, the implementation of FAIMS in the LC Data-Dependent full scan MS/MS analysis of Loperamide and its microsome metabolites revealed a second hydroxylated metabolite which was not found with the ion max alone. This additional metabolite was detected in the base peak chromatogram and subsequently identified due to the significant reduction in chemical noise in the full scan mass spectral data provided by the FAIMS interface.

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