Conclusions: Mass spectral imaging was demonstrated as a complementary technique to quantitative whole body autoradiography.

The sensitivity of the LTQ with MALDI source for tissue imaging application and utility for displaying distribution of particular compounds and metabolites was demonstrated.

The unmetabolized DEHP was not completely eliminated out of the rat in the timeframe of the experiment, and this compound was found mostly concentrated in the intestinal region.

Monitoring a specific metabolite (SRM of MEHP) showed more specificity in the distribution within the rat as compared to the parent, unmetabolized compound.

Parallel experiments, where tissues for the dosed, control, and quantitative whole body autoradiography came from different rats, made comparisons difficult due to variability among animals.

> To download the complete application note, please use the link at the top left of this page.