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  App. Note 437: LC-MS/MS Analysis of Herbicides in Drinking Water at Femtogram Levels Using 20 mL EQuan Direct Injection Techniques
 The goal of this application note is to compare different large volume injections using a loading column and an analytical column with two HPLC pumps.
 

Conclusion:
Using a preconcentration column in tandem with an analytical HPLC column allowed for the quantitation of a triazine herbicide mixture over the concentration range 0.1 – 100 pg/mL. Direct 20 mL injections were performed with the two HPLC columns. The large injection volume capabilities of the EQuan system eliminated the need for laborious and expensive offline preconcentration using solid phase extraction. Injection volumes ranging from 1 mL to 20 mL are possible using this configuration, thus offering flexibility for laboratories based on their sensitivity and reporting requirements.

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   Products used for this Application
  Product #   Product Name   Image  
 IQLAAEGAAXFAGBMAND  TSQ Quantum Access      Select