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| App. Note 444: A Robust and Sensitive Workflow for Label-Free, Quantitative Identification of Differentially Expressed, Endogenous Peptides in Human Serum |
| In this study, we describe the development of a workflow specifically geared toward the efficient recovery and identification of endogenous peptides. |
| This was done from blood utilizing a combination of up-front batch sample prep and on-line liquid chromatography coupled with high-resolution tandem MS using HCD and CID fragmentation on an LTQ Orbitrap XL. Quantitative differential analysis of the endogenous peptides was carried out using label-free analysis with SIEVE™ software algorithm. The novel workflow was applied to investigate the quantitative differences in endogenous peptides recovered from maternal serum in
normal and Trisomy 21 (Down’s Syndrome) pregnancies
To read/download the complete pdf of this note, please use the link on the left side of this page.
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