This includes an increase in identification of lower intensity precursors, when compared to existing state-of-the-art technologies.

• The LTQ Velos ion trap identified ~240% more proteins and ~130% more unique peptides from a highly complex sample than did a Q-TOF , where >90% of the proteins identified by the Q-TOF were also identified by the LTQ Velos.

• The LTQ Velos dual-pressure ion trap demonstrated higher sensitivity for samples at lower levels, with more than a 150% increase in the number of identified unique peptides for a low load of 20 ng, versus the LTQ XL ion trap.

• With the increase in the number of identified proteins, the LTQ Velos offered greater access to low-abundance proteins as shown by a 133% increase in the number of identified signal transduction proteins.

• The LTQ Velos dual-pressure ion trap offered an increase in experimental throughput, identifying more proteins and peptides than the LTQ XL ion trap in 1/3 the separation time for a complex sample.

• The better quality of the MS/MS scans in the linear ion traps produced higher Mascot scores for equivalent peptides compared to those of the Q-TOF instrument. Higher mass accuracy of analytical scans acquired with the Q-TOF was beneficial but not sufficient to produce superior peptide identification.

• Increasing the scan rate on the Q-TOF resulted in a decrease in signal and spectral quality for MS and MS/MS scans and a corresponding decrease in the number of identified peptides.

To read/download the complete application note, please use the link on this page.