Introduction
Metanephrine (MN) and normetanephrine (NMN) (Figure 1) are created by the action of catechol-O-methyl transferase on epinephrine and norepinephrine, respectively. Current clinical research methods usually involve labor-intensive, time-consuming offline sample preparation. In this study, a sensitive, selective LC-MS/MS method was developed using the Thermo Scientific Aria  TLX-1 system powered by TurboFlow™ technology for online sample extraction coupled with the Thermo Scientific TSQ Vantage triple quadrupole mass spectrometer. The results demonstrate the method’s suitability for clinical researchers to measure MN and NMN.

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