The global food market has become more competitive and equally cost responsive. The need for analytical procedures that permit high sample throughput as well as higher sensitivity allied to good reproducibility is growing by the day.1,2,3 A method using automated online extraction with tandem mass spectrometry is presented as an alternative to the commonly used, time-consuming solid-phase extraction (SPE) method. Quinolones, including fluoroquinolones, are a group of synthetic antibacterial compounds used in the treatment of several diseases. There has been a significant and progressive increase in the use of quinolones in animal production, which has led to their residual presence in food. In the European Union, the maximum residue limits (MRLs) for several of these compounds are defined for different food matrices of animal origin, but not for honey.4 Furthermore, the presence of these compounds is an indication of unsafe practices of food production and deficient methods in the production of honey. The complexity of the matrix plays a fundamental role on the adoption of the method of analysis. Thermo Scientific TurboFlow technology enables the reduction of sample preparation as well as the elimination of interferences from complex matrices such as honey.