| Characterization of biologically active lipids and their metabolites, or lipidomics, is a growing area of research that is complementary to proteomics and metabonomics approaches. However, unlike most components in proteomics and metabonomics, understanding the biological activity of several lipids requires measurement of the different stereoisomers of those lipids.
This application report describes the methodology and preliminary results, where normal-phase chiral liquid chromatography is employed to separate the enantiomers of hydroxy fatty acids produced from arachidonic acid and linoleic acid by lipoxygenases and cyclooxygenases.1 High-sensitivity detection of these lipid metabolites is possible using derivatization with the pentafluorobenzyl (PFB) moiety2 and employing electron capture atmospheric pressure chemical ionization (APCI), followed by analysis using selected reaction monitoring (SRM) on a triple quadrupole mass spectrometer.
To view the complete application note, please download the PDF file on the left of this page. |
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