| The development of an MS/MS method for a list of target compounds can be
divided into six steps:
• Step 1: Specifying segment breaks
• Step 2: Selecting the precursor ion
• Step 3: Setting the Q value
• Step 4: Examining isolation efficiency
• Step 5: Optimizing Collision Induced Dissociation (CID) voltage
• Step 6: Defining product scan range
These steps are outlined in this application, with the use of the PolarisQ ion trap GC/MSn and HighChem Mass FrontierTM 4.0, a
software package for the management, evaluation and interpretation of mass spectra.
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