This report demonstrates the role of the TSQ Quantum Discovery in carrying out in vitro studies of compound metabolic stability and identification of Phase I and II metabolites. For the determination of compound metabolic stability, samples were quantified using SIM and from the metabolic turnover an in vitro half-life was calculated. Full-scan MS/MS data dependent scans were carried out for the identification of Phase I metabolites.

For phase II metabolites, Constant Neutral Loss (CNL) scans followed by data dependent triggered MS/MS analysis were carried out for identification and structural elucidation.

To read/download the complete application, please select the link on the top-left of this page.