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  App. Note 360: Increased Coverage in the Transmembrane Domain with Activated Ion ECD for Top-down FTMS of Integral Membrane Proteins
 Top-down analysis of of Integral Membrane Proteins using the LTQ FT is demonstrated.
 This application note describes experiments demonstrating a top-down analysis of the c-subunit of the ATP synthase (ATPH) from chloroplasts of Arabidopsis thaliana, an integral membrane protein with two transmembrane domains. The experiment was conducted (see Thermo Scientific Application Note 30081) using a LTQ FT equipped with electron capture dissociation (ECD) to achieve a consistent mass accuracy of better than 5 ppm for product ions from the protonated protein molecules.

To read/download a PDF of the complete application note, please use the link on the left of this page.

   Products used for this Application
  Product #   Product Name   Image  
 IQLAAEGAAPFADAMAII  LTQ FT Ultra Hybrid Mass Spectrometer      Select
 
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  Name
  Proteomics