| FT-SPR allows SPR to be measured on an FT-IR platform, where FT-IR multiplexing and wavelength precision features are expected to result in key technical advantages over conventional angle-shift based SPR. FT-SPR measurement is performed at a fixed angle of incident light, and reflectivity is measured over a range of wavelengths in the near-infrared.2 In FT-SPR, the minimum in reflectivity occurs at the “SPR wavenumber.”
Since FT-IR spectrometers typically have high wavenumber resolution and a wide range of reflection angles are available in an SPR™100 module from Thermo Fisher Scientific, FT-SPR is a highly sensitive method with remarkable dynamic range for surface analysis.
This application note is based on the use of the innovative FT-SPR detection technique that couples the SPR 100 module to a FT-IR spectrometer. To demonstrate the broad utility of FT-SPR analysis, this note details how FT-SPR systems are used to monitor: 1) nucleic acid binding to surface-immobilized oligonucleotides; and 2) antibody binding to surface-immobilized peptides. |
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