| The analysis of fatty acids is generally performed using GC-MS methods. While these methods provide the necessary sensitivity, sample preparation involves derivatization, which is time consuming and labor intensive. In comparison to GC methods, fatty acid analysis by LC-MS is simplified because derivatization is not required. The resulting LC-MS chromatogram, however, often exhibits a high chemical background that ultimately limits detection.
In this work, the gas-phase separation of high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) is used in combination with LC and zero neutral loss tandem MS to increase the selectivity of the method.
The chemical background in the resulting LC-FAIMS-SRM chromatograms is significantly reduced with respect to that collected using the LC-SRM method. The result is improved detection limits for fatty acid analyses. To read/download the complete application note, please download the file on this page. |
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