The approach presented here provides a sensitive and selective method for preparing and analyzing horse plasma for the presence of rhEPO or DPO. The advantages of this method include the ability to use up to six diagnostic peptides to confirm or refute the presence of either illegal protein-based drug.

The use of stable-isotope labeled analogues provides further means of confirming the presence of diagnostic peptides based on chromatographic retention times and ion ratios.

The sensitivity demonstrated enabled detection up to 72 hours following the last administration of rhEPO, increasing the confidence that the described method is useful in the racing industry to maintain a level field of competition.

Of particular interest is the measured sensitivity that was achieved using microspray, increasing the analysis.

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