The complete GUS method described in this note utilizes either an LXQ or LCQ Fleet ion trap and includes an SPE procedure and two LC methodologies which allow for the identification of over 275 compounds in human urine. The 30 minute LC method is suggested for the most thorough investigation of all analytes present in the sample, while the 13 minute method is recommended for laboratories that operate in environments which require short turn around times. Accompanying ToxID software allows for automatic data analysis and reporting, thereby eliminating the need for manual data interpretation and increasing confidence in compound identification. It is worth noting that when compared to other general unknown screening methods, both the 13 and 30 minute LC-MS based methods identify more analytes.

An important feature of the GUS workflow is the ease of adding new analytes to the screening method, highlighted in Table 6. This aspect of the application is very important for general unknown screening, where new target compounds are continually being added to the target list.

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