Goal: To present a workflow for sample re-injection that employs a mass defect filter and time-based datadependent accurate mass lists to trigger MSn scans for potential metabolites in bile.

Conclusion: A strategy for re-interrogation of “in vivo” metabolite samples is demonstrated in this application note. By  using a mass defect filter, component detection with MSn, and time dependent accurate mass inclusion lists, MSn spectra were triggered for several lower-level metabolites.

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