Purpose: To show a complete analytical metabolomic workflow including (1) data acquisition using a high resolution accurate mass instrument that is equipped with a Higher Energy Collisional Dissociation (HCD) cell and coupled to a high pressure LC (Figure 1), (2) metabolite differential abundance analysis, and (3) structural elucidation of relevant metabolites using accurate mass and HCD fragmentation information to highlight the component differences between green and black tea.

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