Recently, we have demonstrated the utility of high-resolution selected reaction monitoring (H-SRM) method on a triple quadrupole instrument for highly selective and sensitive quantitation of targeted peptides in complex matrices. In this study, a targeted peptide quantitation assay is created for a triple quadrupole mass spectrometer to determine the natural abundance of six peptides in sixteen samples from the salivary gland secretions of eight donors. In order to get absolute quantitative information from the targeted peptides, isotopically labeled peptides were used as internal standards.

To increase the detection capabilities of the assay, an H-SRM approach was employed using the Thermo Scientific TSQ Vantage triple quadrupole mass spectrometer in which the mass resolution on the first quadrupole (Q1) was set to 0.4 FWHM.

To read/download the complete note, please use the link on this page.