The novel large volume splitness injection technique, coupled with a sequential full scan/SIM acquisition, can expand that role and consequently increase the value of GC/MS as an analytical tool. By combining an injection technique that allows injection of up to 50 µL of sample with a sequential full scan/SIM acquisition, the forensic scientist can achieve several objectives with a single injection using the Thermo Scientific DSQ series single quadrupole GC/MS system. SIM analysis enables confirmation of pre-screened presumptive positives, while the presence of full scan data allows investigation into other compounds of interest that may be present in a sample.