PSB126 Merging CID and ETD Database Search Results to Increase Confidence in Protein ID

Electron transfer dissociation (ETD) is an activation method that is complementary to CID. It not only favors multiply charged peptides, but also preserves labile post translational modifications (PTMs). Recent studies indicate that increased identification confidence is obtained using a combined CID and ETD approach for peptide / protein identification

PSB122 Using Multistage Activation in an Ion Trap Mass Spectrometer

Data Dependent™ Neutral Loss MS3 (DDNLMS3) analysis enables selective fragmentation

PSB121 Enhanced Fragmentation of Small Molecules in a Thermo Scientific LTQ™ Linear Ion Trap Mass Spectrometer Using Stepped Normalized Collision Energy

The Stepped Normalized Collision Energy (SNCE) process applies several different Normalized Collision Energy “steps” to a sample during ion dissociation

Data Dependent acquisition using Ion Trap mass spectrometers allows automated real-time decisions for subsequent MSn analysis

To achieve maximum benefit in an LC/MSn experiment, the series of events starting from the process of filling the ion trap through detecting the ions must be optimized for a chromatographic timescale

An application of Data Dependent™constant neutral loss (DDCNL) scanning to identify in vitro metabolites of glyburide is illustrated

Post-translational modifications, such as phosphorylation and glycosylation, are associated with regulation, location and function of proteins

To demonstrate the capabilities of isotope pattern searching, in-vitro metabolism of diazepam was studied using LC/MS in conjunction with Metabolite ID 2.0 software

The sensitivity and selectivity of electrospray mass spectrometry, combined with the on-line sample concentration and desalting capabilities of reverse-phase HPLC, creates a tandem system of choice for the automated, high-throughput analysis of compounds in a mixture

As a peak elutes and its intensity crosses the threshold, the on-board processor in the LCQ™ determines the most intense ion and immediately initiates an MS2 scan. In this way, both MS and MS2 spectra are acquired on each component as it elutes

For the first time MSn spectra are now as reproducible as electron impact spectra. The combination of Normalized Collision Energy with WideBand Activation™ means that spectral libraries can be built, searched and exchanged

The resonance excitation process used for MSn in an ion trap device fragments only the selected ion