Data Dependent™ Neutral Loss MS3 (DDNLMS3) analysis enables selective fragmentation

The Stepped Normalized Collision Energy (SNCE) process applies several different Normalized Collision Energy “steps” to a sample during ion dissociation

Data Dependent acquisition using Thermo Scientific Ion Trap mass spectrometers allows automated real-time decisions for subsequent MSn analysis

To achieve maximum benefit in an LC/MSn experiment, the series of events starting from the process of filling the ion trap through detecting the ions must be optimized for a chromatographic timescale

Post-translational modifications, such as phosphorylation and glycosylation, are associated with regulation, location and function of proteins

To demonstrate the capabilities of isotope pattern searching, in-vitro metabolism of diazepam was studied using LC/MS in conjunction with Metabolite ID 2.0 software

The sensitivity and selectivity of electrospray mass spectrometry, combined with the on-line sample concentration and desalting capabilities of reverse-phase HPLC, creates a tandem system of choice for the automated, high-throughput analysis of compounds in a mixture

As a peak elutes and its intensity crosses the threshold, the on-board processor in the Thermo Scientific LCQ™ determines the most intense ion and immediately initiates an MS2 scan. In this way, both MS and MS2 spectra are acquired on each component as it elutes

For the first time MSn spectra are now as reproducible as electron impact spectra. The combination of Normalized Collision Energy with WideBand Activation™ means that spectral libraries can be built, searched and exchanged

The resonance excitation process used for MSn in an ion trap device fragments only the selected ion