The LTQ Orbitrap XL with New HCD Collision Cell Provides:


• Excellent sensitivity and fragmentation efficiency
• Reliable identification and quantitation in one experiment
• More confidence in biomarker research

Base peak chromatogram of nano LC-MS and MS/MS analysis.

An important aspect of proteomics is not only the identification of all proteins in complex biological samples but also the accurate determination of their relative concentrations. Several analytical approaches to protein quantitation have been developed, including isotopic labeling techniques such as iTRAQ™. This method relies on the measurement of specific reporter ions in the low m/z region of MS/MS spectra of target peptides. The new HCD collision cell of the LTQ Orbitrap XL is ideally suited to perform such experiments with excellent sensitivity and signal-to-noise ratio (S/N) for the reporter ions and the fragment ions of the peptide sequence.

Experimental Method


• Mouse cell line
• iTRAQ labeled
• Online nano LC-MS and MS/MS
• Orbitrap mass analyzer full scan at R = 30,000
• Data Dependent™ Top 3 Orbitrap MS/MS using HCD
• Dynamic exclusion

HCD spectrum for FSALESQLQDTQELLQEENR (MYH9_MOUSE, Myosin-9)
in mouse cell line digest derivatized with four iTRAQ reagents.