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The new Thermo Scientific LTQ Orbitrap Discovery™ provides
access to the breakthrough Orbitrap™ technology for a wide range of
labs. The robust, powerful and cost-effective system is ideally suited for
general proteomics and metabolism applications, offering a superior alternative
to Q-TOF systems. Featuring high resolution and excellent accurate mass
capabilities, this hybrid LC-MS system achieves high throughput label-free
profiling for differential expression. Its MSn capability makes the instrument
ideal for small molecule characterization and structural elucidation. The LTQ
Orbitrap Discovery is a highly sensitive system with up to 30K resolving power
and fast scan rate, enabling the separation of trace level, isobaric
analytes.
| Principle Of
Operation |
| The LTQ Orbitrap Discovery mass spectrometer is a
hybrid system combining the LTQ XL linear ion trap mass spectrometer and the
Orbitrap mass analyzer. Ions generated in the API ion source are trapped in the
LTQ XL. | Once trapped in the LTQ XL, the ions are analyzed using the MS
and MSn scan modes. Then, the ions are axially ejected from the LTQ XL and
collected in a C-shaped ion trap (CTrap) from which they are passed into the
Orbitrap mass analyzer. | The ions transferred from the C-Trap are
captured by rapidly increasing the voltage on the center electrode of the
Orbitrap. The trapped ions assume circular trajectories around the center
electrode and their axial oscillations, along the center electrode, are
detected. |
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