Human Mesenchymal Stem Cell Protocol: Adipogenic Differentiation
An AdvanceSTEM™ Adipogenic Differentiation Kit has been developed to support the Adipogenic differentiation of human Mesenchymal Stem Cells isolated from adipose tissue and bone marrow.

Human Mesenchymal Stem Cell Protocol: Chondrogenic Differentiation
A Chondrogenic Differentiation Kit has been designed to support the chondrogenic differentiation of human Mesenchymal Stem Cells isolated from bone marrow and adipose tissue.

Human Mesenchymal Stem Cell Protocol: Cryopreservation
A Cryopreservation Medium has been developed to support the cryopreservation of a variety of adherent cell types, including human Mesenchymal Stem Cells (hMSCs), Multipotent Cord Blood Unrestricted Somatic Stem Cells (MCBUSSCs) and human Amniotic Epithelial Stem Cells.

Human Mesenchymal Stem Cell Protocol: Neural Differentiation
An AdvanceSTEM Neural Differentiation Kit has been developed to support a variety of human somatic stem cells into neural cells. These include: Adipose-Derived MSCs, Bone Marrow Derived MSCs and Multipotent Cord Blood Unrestricted Somatic Stem Cells.

Human Mesenchymal Stem Cell Protocol: Osteogenic Differentiation
An Osteogenic Differentiation Kit has been designed to support the osteogenic differentiation of human Mesenchymal Stem Cells isolated from adipose tissue and Multipotent Cord Blood Unrestricted Somatic Stem Cells.

Human Mesenchymal Stem Cell Protocol: Oil Red O Staining of Adipogenic Cultures

Human Mesenchymal Stem Cell Protocol: Subculturing hMSCs
Human Mesenchymal Stem Cells (hMSC's) are primary cells that can be successfully cultured approximately eight passages.

Human Mesenchymal Stem Cell Protocol: Thawing and Plating
Human Mesenchymal Stem Cells (hMSC) and Multipotent Cord Blood Unrestricted Somatic Stem Cells (MCBUSSC) are primary cells which can be successfully cultured for approximately five to six passages.

Mouse Embryonic Feeder Cell Protocol: Cryopreservation of MEFs
Mouse embryonic fibroblasts (MEFs) are the most commonly used feeder layer when culturing either mouse or human embryonic stem cells (ESCs). MEFs provide a complex, unknown mixture of nutrients and substrata for long term growth and proliferation of undifferentiated ESCs.

Mouse Embryonic Feeder Cell Protocol: Mitotic Inactivation by Mitomycin C
Mouse embryonic fibroblasts (MEFs) require careful culture and maintenance. Keeping MEFs in a healthy proliferating state, producing all of the matrix and growth factor support for embryonic stem cells (ESCs), is an important goal.

Murine Embryonic Stem Cell Protocol: Primogenix PRX-129/X1 ES Cells
These are classic mouse embryonic stem cell (mESC) culture conditions. The ES cells are cultured in medium containing DMEM, FBS, LIF and co-cultured with primary mouse embryonic fibroblasts (MEFs).

Murine Embryonic Stem Cell Protocol: Primogenix PRX-B6N ES Cells
Pluripotent PRX-B6N ES cells have a very distinct morphology when cultured under the following conditions, growing as tightly clustered colonies with smooth phase bright borders. Primogenix B6N ES cells grow quickly and require daily maintenance.

Mouse Embryonic Feeder Cell Protocol: Subculturing MEFs
Mouse embryonic fibroblasts (MEFs) require careful culture and maintenance. Keeping MEFs in a healthy proliferating state, producing all of the matrix and growth factor support for embryonic stem cells (ESCs), is an important goal.

Mouse Embryonic Feeder Cell Protocol: Thawing Cryopreserved MEFs
Mouse embryonic fibroblasts (MEFs) require careful culture and maintenance. Keeping MEFs in a healthy proliferating state, producing all of the matrix and growth factor support for embryonic stem cells (ESCs), is an important goal.