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A number of markers of oxidative stress can be used to evaluate the effects of exercise or aging on proteins. One of these potential markers, o,o’-dityrosine (DT), can be analyzed by gas chromatography-mass spectrometry (GC/MS) as part of a profile of protein oxidation.
This application describes a method for the gas chromatographic/mass spectrometric analysis of o,o’-dityrosine and its 13C-labeled analog using negative ion chemical ionization. o,o’-dityrosine is evaluated as a potential in vivo marker of cellular oxidation. This method provides optimization techniques and discusses the use of alternate tuning compounds and their effect on the analysis of these compounds.
The analysis of o,o’-dityrosine as a marker of oxidative stress in study subjects by NICI SIM enabled quantitation of the naturally-occurring DT as a marker of cellular response to exercise. |
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