DSQ II with TRACE GC Ultra
ITQ 700 with TRACE
GC Ultra
TSQ Quantum GC
TSQ Vantage with Accela
Transcend System
Exactive LC/MS
LTQ Orbitrap
DFS High Resolution GC/MS with Dual TRACE GC Ultra
and TriPlus Autosampler XT
DELTA V Isotope Ratio
MS with TRACE GC Ultra, GC-C/TC III
Combustion and Autosampler AS3000 |
Screening
and Confirmation for
Performance Enhancing Substances
• Anabolic
Steroids
• Peptides, Hormones, Proteins
• Narcotic
Analgesics, Beta-Blockers
• Diuretics
• Stimulants
Comparison of urinary
T/E ratios and δ13C testosterone values determined by
GC/Isotope ratio MS after ingestion of 40 mg testosterone-undeconate. Values
above this range give direct evidence for synthetic testosterone excreted in
urine (dotted line)
Courtesy of German Sport University Cologne –
Center for Preventive Doping Research / Institute of Biochemistry.
Analysis and confirmation of synthetic
testosterone
The administration of synthetic testosterone is detected
by the testosterone/epitestosterone (T/E) ratio in urine using GC/MS.
Confirmation is performed by GC/Isotope Ratio MS.
While the surplus of
synthetic testosterone is excreted rapidly in the urine, as evidenced by the
fast return of the T/E ratio to a value below 3 (red curve), the carbon atom
13C/12C ratio of testosterone (δ13C)
stays at the value of synthetic testosterone for a much longer period of time
(blue bars) due to supressed production of testosterone. The
13C/12C ratio of synthetic testosterone differs from the
13C/12C ratio of endogenous testosterone. The decrease
back to the normal 13C/12C ratio of testosterone starts
with the recovered production of endogenous testosterone and is delayed by
almost 12 hours.
 Testosterone |
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