Methods: Proteins from neat and HSA-stripped human plasma were digested enzymatically and separated by a two dimensional HPLC, using strong cation exchange and reversed phase chromatography, then analyzed by on-line LC/MS/MS with an ion trap mass spectrometer. Proteins were identified using TurboSEQUEST® software to search observed MS/MS spectra against a human protein database.

Results: 359 proteins were identified with high confidence.

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