Methods: We applied the cleavable isotope-coded affinity tag (ICAT) method as well as a simple proteolyis to human CSF for protein identification via 1-D LC/MS/MS.

Results: The applicability of the ICAT™ method to CSF and migraine-related proteins is limited due to low reproducibility and an overall decrease in the number of proteins observed– leading to an inability to monitor the CSF proteins involved in migraine. In contrast, simple 1-D LC/MS/MS experiments of unmodified CSF digests provide data that can be used to monitor a number of migraine related proteins.

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