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Confident assignment of the charge state of a precursor
from which a particular MS/MS spectrum is derived is crucial for successful and
efficient spectrum analysis. High quality spectra obtained by Electron Transfer
Dissociation (ETD) are usually observed from peptide precursors with charge
states 3+ and higher. In the absence of precursor ion charge state information
from unit resolution full MS spectra, several charge states are assumed for each
precursor ion when a database search is performed. Therefore, uncertainty about
the precursor ion charge state of an ETD spectrum requires that each spectrum be
searched several times in order to cover several potential precursor charge
states (usually from 2+ to 7+). A new ETD data pre-processing function was
developed to automatically assign charge states to precursor ions according to
the characteristics of ETD spectra, and is now included in BioWorks™ software
version 3.3.1. |
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