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Inside a cell there are thousands of different proteins at work at any given time. To understand the function of a particular protein in detail, the protein must first be isolated and purified. There are a number of different ways to separate proteins that are in complex mixtures such as cell fluid. Proteins can be separated on the basis of differences in their charge, size, shape, reactivity, somatic activity, or any structural element unique to proteins that a researcher is trying to isolate.
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