| Please send
Comments & Suggestions to criticalmass@thermo.com |
| MSeminar |
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Introducing MSeminar, Thermo's Mass Spectrometry
Webinar Series.
Featuring the following Seminars in
September:
Introduction to the Finnigan ProteomeX LTQ:
Methods and Applications
Finnigan TSQ Quantum for
Quantitation: An Introduction
Environmental Applications
for the Finnigan TSQ Quantum: Chloramphenicol, Perchlorate
& CDFA
Finnigan LTQ FT: The Most Advanced LC-MS and
MS/MS System Ever
Participate
in the MSeminar that interests you |
| Article: Top
Down Proteomics |
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Protein analysis and identification via mass
spectrometry is performed by either the Top-Down or the
Bottom-Up approach.
Analysis of intact proteins by the
Top-Down approach allows avoiding digestion, additional
separation steps, and sample loss; although desalting is
necessary in many cases.
The requirement for such an
analysis is high resolution at fast scan repetition rate in
combination with high mass accuracy. This is provided by the
Finnigan LTQ FT which is a combination of a linear ion trap
and an ICR analyzer.
Using electrospray ionization on the
Finnigan LTQ FT samples of intact proteins applied to analysis
result in multiply charged molecular ions (see figure)
observed in the normal scan range of the instrument (m/z
400-4000).
Multiply charged species can therefore be
isolated in the linear ion trap and fragmented in a data
dependant manner in both the ion trap part (CID) or in the I!
CR cell using ECD or IRMPD or even the simultaneous
combination of both. Also the ions can be detected with either
the ion trap detection system or with the ICR cell detector
utilizing high resolution and high mass accuracy. ...more |
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| Proteomics News |
Attend
our Free European Proteomics Seminar Series.
Attend
our European Proteomics Courses.
Watch
the new vMALDI Video.
Download
Proteomics Pioneers Vol2. including interviews with
experts: Catherine Fenselau, Jonathan Weissman, Richard
Caprioli, John Yates, Gary Siuzdak, Alan Marshall, Bill
Hancock, Marvin Vestal, Norm Dovichi, Mike Snyder, Don Hunt,
Julio Celis and John Bergeron.
Be sure to visit Thermo at
the 15th
Conference of Methods in Protein Structure Analysis. |
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| Trap Talk:
WideBand Activation |
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WideBand Activation operates by applying resonance
excitation energy over a mass range which extends to 20 amu
lower than the selected ion. This ensures that both the parent
ion and any subsequent water loss ion will undergo
fragmentation while retaining the specificity of the unit mass
isolation of the parent ion.
Figure shows the MS2 spectrum
of Labetalol acquired with and without WideBand Activation.
Standard activation conditions, even with high collision
energy, show only the water loss ion. WideBand Activation, in
contrast, achieves meaningful fragmentation data even at low
collision energies.
WideBand activation thus effectively
eliminates an additional stage of MS in MSn experiments.
WideBand Activation is available on all Finnigan LTQ and LCQ
series instruments running under Xcalibur control. ...more.
In the n! ext Issue: Dynamic Exclusion. In the previous Issue:
Normalized
Collision Energy. |
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| Need a Better
Detector for your HPLC? |
|
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Featuring the Finnigan LCQ Advantage Max - Take
your HPLC to New Heights
Including a preview of a soon
to be published Application Note on identifying metabolites in
complex biological samples!! |
| Scientist
Profile |
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Daniel
C. Liebler, Ph.D.
Professor of Biochemistry and
Pharmacology
Director, Proteomics Laboratory, Mass
Spectrometry Research Center
B.S., Chemistry, Villanova
University
Ph.D., Pharmacology, Vanderbilt
University |
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| Through the
Customer's Eyes |
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"...We
expect the LTQ to become a workhorse for our biomarker
discovery effort for many years to come."
-- Indiana
Centers for Applied Protein Sciences (INCAPS) |
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