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| Tip
of the month: How to clean the Ion Transfer
Tube |
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Submerse the Ion
Transfer tube in a solution of 1% by volume nitric acid in
water (HPLC grade) and sonicate for 5 minutes. Remove the Ion
Transfer tube and thoroughly rinse with water. Sonicate the
Ion Transfer tube in HPLC grade methanol for 15 minutes,
remove and dry with a stream of nitrogen. |
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| Proteomics
Application: Top Down Analysis of Yeast
Proteins |
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| Protein
identification via mass spectrometry (MS) currently relies on
two general strategies, namely, the bottom-up and top-down
approaches. With the top-down approach, the intact proteins,
individually or in mixtures, are mass analyzed and then
fragmented inside the mass spectrometer without prior
enzymatic digestion. The advantages of this method in
comparison to the bottom-up approach are the ability to
measure the intact protein molecular weight, thus preserving
both the protein sequence and the integrity of
post-translational modifications. Currently, the top-down
approach has been almost exclusively restricted to FTICR
instruments due to the need for high resolving power and mass
accuracy. |
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| Download Full
Article (PDF 399kb) |
| Quantum Waves:
Identification of Phase-I Metabolites |
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The
identification of biotransformation products of a drug is
important in understanding its pharmacological and potentially
toxicological effects. In order to improve the efficacy of a
drug and minimize any toxic side effects, drug candidates are
being screened earlier in the discovery process. By
identifying metabolites of a drug candidate, the compound may
be modified to block metabolically active sites increasing its
duration of action, or the structure can be altered to prevent
the formation of toxic by-products. One of the common means of
identifying drug metabolites by LC/MS/MS is employing the
selective scan modes of a triple quadruple mass spectrometer,
namely the parent ion scan... |
| Download Full
Article (PDF
253kb) |
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| ASMS 2005 Events from
Thermo |
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Thermo has several events
planned for ASMS 2005, including:
User Meeting: This
application-focused event will take place Sunday morning June
5th beginning from 7:30PM to 12:00PM.
Annual Customer
Event: This event will take place immediately after the
morning User Meeting.
Lunchtime Sessions: These
sessions will take place Monday through Wednesday June 6th-8th
from 12:30PM to 1:20PM
Hospitality Suite: The suite
will be open Monday through Wednesday June 6th - 8th from
6:30PM - 9:30PM. |
| More
Information |
|
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| Breakthrough
Technology at ASMS |
|
Learn more about Thermo's
new breakthrough accurate mass solution for small molecule
analysis: Dr. Stevan Horning will be talking about
Thermo's Revolutionary New High-Resolution Small Molecule
Solution at the Users' Meeting on Sunday, June 5th in the Rio
Grande Ballroom.
The presentation will focus on:
Metabolite identification using accurate mass and MSn
high-resolution, accurate mass component ID in complex
matrices with routine high-resolution, high-throughput
accurate mass operation and more.
Mark your calendar
now!! |
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| Quantum Waves at
ASMS |
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| Realize up to eight times
improvement in sensitivity for your H-SRM assays. Visit our
hospitality suite & find out what's new on the
Finnigan™ TSQ™ Quantum Ultra. |
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| Small Molecule
Application: Rapid Quantitative Screening of
Drugs |
|
| Drugs of abuse
in the horse racing industry encompass a variety of chemical
classes and are typically analyzed from a complex urine
matrix. These factors render the rapid and effective
diagnostic screening of these drugs at low levels difficult.
This article discusses developing a simple and fast, yet
rugged LC/MS based method capable of simultaneous qualitative
and quantitative analysis. The authors of this article have
evaluated the application of the Finnigan LTQ™ linear ion trap mass
spectrometer for providing low levels of... |
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| Download Full
Article (PDF 420kb) |
| Software:
Xcalibur™ 2.0 and XReport
1.0- Easy, Stable, Compliant, Versatile |
|
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Version 2.0 of
Xcalibur continues the tradition of a very versatile,
easy-to-use data system - now enabling 21 CFR part 11
compliance. Whether you are an experienced Xcalibur user or
new to our software, Xcalibur's homepage offers easy
navigation through the process of instrument setup, sequence
setup, and data acquisition. The review of results is easy in
one of three browser environments; Qual Browser, Quan Browser,
Library Browser. Reporting has gotten easier with the all-new
XReport. |
| Read more on
XReport |
| Trap Talk:
Determining Metabolic Stability |
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| A high
throughput strategy is essential in accelerating the lead
optimization phase in the drug development cycle. The strategy
discussed here involves the simultaneous determination of the
nature and extent of metabolism by performing metabolite
identification and metabolic stability measurements in a
single analytical run. Using the Finnigan LTQ, the increased
throughput gains are achieved without sacrificing data
quality. The combination of ultra-fast cycle time and high
spectral quality on the LTQ are key to achieving a balance
between speed and spectral quality. |
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| Read Full
Article |
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