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| Visit us at ABRF 2006
(Booth #114), Long Beach, CA |
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Learn more about the
breakthrough LTQ Orbitrap™ hybrid mass spectrometer and
Thermo's proven solutions for proteomics:
• Label-Free
Biomarker Discovery & Validation
• Top-Down Protein
Isoform Analysis
• High-Throughput Protein ID
•
Quantitative Proteomics
• Ultra-Sensitive PTM
Characterization
• Superior Fragmentation with PQD, ETD |
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Register for one of Thermo's
Workshops |
| Tip
of the Month: Maintaining API Probes |
|
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The API probes
for the Ion Max ion source are robust and require minimal
maintenance. Here is a quick solution in case of an
obstruction caused by salt precipitation: Flush the probe with
50:50 HPLC-grade Methanol : distilled water solution through
the LC. |
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| Proteomics: De
novo Sequencing with the Finnigan LTQ
FT |
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| The extensive use of
large-scale genomic sequencing has greatly simplified the task
of identifying proteins of many organisms by mass
spectrometry, since open reading frames in the nucleotide
sequence can be used as templates to deduce the sequence of
corresponding proteins. The masses of peptides produced by
proteolytic degradation of an unknown protein can be compared
with those that are calculated by in-silico degradation of the
proteins in the database. This ‘mass mapping’ is often
sufficient to identify... |
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| Download Full
Article [PDF 127
kB] |
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| 2006-2007 Thermo Wall
Calendar |
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| Request your free
2006-2007 Thermo wall calendar. |
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Now |
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| 2006-2007 Columns and
Consumables Catalog |
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| Request your copy of the
2006-2007 consumables catalog. |
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Now |
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| Quantum Waves:
Detecting Glucuronic Acid Metabolites in Liver
Microsomes |
|
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Glucuronidation
is an important conjugation for the metabolism of xenobiotics
and is catalyzed by various uridine diphosphate
glucuronosyltransferases. It is considered to be an important
detoxification pathway. Hydrophilic glucuronic acids are
eliminated from the body via excretion in bile or urine. In
vitro tools for predicting in vivo clearance of drugs, which
are predominantly glucuronidated, are rarely determined at
early drug discovery stages. This work describes methods to
study the glucuronidation rate of New Chemical Entities using
liver microsomes. |
| Download Full
Article [PDF 112 kB] |
| Small Molecule
Seminars - Coming Soon to a City Near You |
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| Register now to attend
informative sessions on compound identification and
bioanalysis. The new Finnigan™ LXQ™ and LTQ Orbitrap mass
spectrometers deliver more confident compound identification
faster, utilizing Data Dependent™ techniques and accurate mass
capabilities, respectively. The sessions will also cover two
software packages that enable small molecule quantitation:
QuickQuan for automated high-throughput discovery quantitation
and LCquan™ 2.5 that provides 21 CFR Part 11 compliance with
seamless integration to Watson LIMS™. |
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| Learn More and
Register Now |
| Metabolomics
Scientific Meeting |
|
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Metabolomics
society presents Metabolomics 2006, the second scientific
meeting of the Metabolomics society in Boston, USA. Topics
include metabolomics technologies, metabolomics in healthcare,
lipid maps and lipidomics, plant metabolomics, databases,
structures and informatics, environmental metabolomics,
etc. |
| Learn
More |
| Trap Talk: Stepped
Normalized Collision Energy |
|
| Ion fragmentation is a
crucial tool for structural elucidation of a variety of
compounds in an ion trap mass spectrometer. The standard
technique for ion fragmentation is Normalized Collision
Energy™ (NCE) which improves fragmentation efficiency over a
wide mass range, making it possible to obtain the maximum
amount of structurally relevant information. Stepped
Normalized Collision Energy (SNCE) is an enhancement to NCE
for MS/MS analysis on mixtures of compounds where a variation
in collision energies is required. |
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| Download Full
Article [PDF 59
kB] |
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