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| Tip
of the Month: Real - Time Modifications on Xcalibur™ with LTQ
2.0/2.2 Instrument Control Software |
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Real-Time
Modifications* allows for the modification of select method
parameters during acquisition. It is useful for initial method
development and instrument tuning. Note that the option must
be checked under the LTQ or LXQ tab in instrument setup.
Changes made are tracked in the "Error Log" of RAW files.
*
This option must be selected during software
installation. |
| See
How |
| Webinar: Integrated
Method for Quantification and Identification of Radiolabeled
Metabolites |
|
| Dr. Weiping Zhao from
Bristol-Myers Squibb demonstrates an integrated approach to
utilizing sensitive radioactive profiling and comprehensive MS
analyses off-line using MSC and chip-based nanoelectrospray
FTMS for rapid quantification and characterization of
radioactively-tagged metabolites. |
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| View Webinar
Recording Now [25
Mins.] |
| Metabolomics with LTQ
Orbitrap™ and SIEVE™ |
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Metabolomics
represents considerable promise for correlating phenotypic
changes with the underlying chemical composition when
comparing relative composition and concentration differences
between a control and modified sample set. One of the greatest
challenges for metabolomics studies involves identifying small
changes within a highly-complex matrix. This article discusses
the effect of very high resolution (and ultra-high pressure)
chromatography and high mass... |
| Download Full
Article [PDF 172 kB] |
| Software: Protein
Identification with BioWorks 3.3 |
|
The 3.3 release of
BioWorks, now available from Thermo, includes these following
capabilities:
- A probability-based scoring system
-
PepQuan for quantitation using SILAC™, ICAT™, and iTRAQ™
-
Unified Search Result File (SRF)
- Support for Accurate
Mass searching
- Ability to create and search reverse
databases
- Support for datasets from the vMALDI source and
LTQ XL
- Batch processing with SEQUEST® Queue and Batch
Search
- Easy data conversion to mzData file format
*
ICAT and SEQUEST are registered trademarks of the University
of Washington. SILAC is a trademark of Invitrogen Corporation.
iTRAQ is a trademark of Applera Corporation. |
 |
| Learn more about
this new release of BioWorks. Request a copy of the Brochure
now. |
| Proteomics: Analysis
of Complex Sample of Bacterial Whole Cell
Lysate |
|
| Haemophilus ducreyi is a
gram-negative bacterium and a member of the Pasteurellaceae
family. It is the causative agent of the genital ulcer disease
chancroid. A detailed understanding of the pathogenesis of
chancroid is an important research priority as chancroid has
been shown to be an important cofactor in the heterosexual
transmission of HIV. The genome of a human passage derivative
H. ducreyi strain 35000 has recently been sequenced and is now
publicly available. In the early 2000’s, the elucidation of H.
ducreyi proteome was initiated using the classical approach
via 2D-gels... |
 |
| Download Full
Article [PDF 1,784
kB] |
| Proteomics Seminar
Tour - Europe, Oct 10 - 26 |
|
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Attend one of
Thermo's 2006 proteomics seminars to learn more about:
-
Definitive PTM analysis and improved protein coverage with ETD
technique on the Finnigan™ LTQ™ linear ion trap
- Top-down
protein analysis with the LTQ FT Ultra and ProSightPC™
-
Enabled stable isotope labeling quantitation methods with the
LTQ Orbitrap and BioWorks 3.3
- Label-free differential
expression analysis using SIEVE package
- Tissue imaging
with the Finnigan vMALDI™ source |
| Learn more and
register now |
| Trap Talk: IRMPD and
ECD on FTICR |
|
| The study of in vivo
folding of proteins into their biologically active forms has
been a mainstay of research for decades. Protein folding in
the gas phase has an often underestimated influence in the
recently emerged field of top - down proteomics with ECD:
Tight folding prevents efficient cleavage by ECD and therefore
limits its use. The common result of the application of an ion
activation method prior to ECD is the significant increase of
bond cleavages observable in ECD product ion spectra. This
results in a more complete determination of the protein
sequence in top-down proteomic approaches. This article
describes the use of IRMPD as an activation method prior to
ECD on the protein ubiquitin, a small (8.6 kDa) protein. |
 |
| Download Full
Article [PDF 411
kB] |
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