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Life Sciences Mass Spectrometry
| Tip of the Month: Optimizing H-ESI Source Conditions for FAIMS Analysis |
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FAIMS analysis works best with dry ions. To ensure the ions are dry, the following H-ESI source probe positions need to be optimized:The user needs to ensure that the H-ESI probe is not spraying too close to the entrance plate opening. Typically, the probe is positioned more than 1.5 mm from the entrance plate opening. Usually, the farther back the probe the better, but placement is compound, mobile phase, and liquid flow rate dependent. Tuning is always started with the H-ESI probe in the C or D position, centered, and as far back as possible. The micrometer can be used to bring the probe closer to achieve the maximum stable signal.
Download Quick Reference Guide [PDF 1.35 MB]
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| Metabolomics Case Study: High-Throughput LC/MS Analysis |
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This case study presents an unbiased look at the effects of oxidative stress on the body using the Thermo Scientific LTQ FT™ and Genedata's Expressionist software. Research in most of the "-omics" fields usually follows a familiar pattern. First there are small, targeted studies, as scientists explore the new area with limited tools and data. Then, a technological breakthrough, such as higher sample throughput or improved mass accuracy, enable larger, unbiased studies that begin to reveal the true underlying biology. The success of a metabolomics study by Emory University's Dr. Dean Jones may be a sign that lab technology is finally ready to handle the size and complexity of the human metabolome.
Download Full Case Study [PDF 819 kB]
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In collaboration with Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Emory University, Atlanta, Georgia, USA
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Mass Spectrometry User Meeting (Oct 7-8, 2008, North America)
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Please join us for a day of applications-focused presentations highlighting new mass spectrometry developments, tips and techniques for proteomics, metabolism and quantitation. Presenters will convey best practices for optimization and utilization of Thermo Scientific LC/MS hardware and software solutions.
Learn More and Register
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| Forensic Toxicology: Identification of Psychotropic Substances in Mushrooms and Chocolate by UHPLC/MS |
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The identification of controlled substances in food matrices is a challenge for forensic laboratories. Classical techniques, such as color tests and thin-layer chromatography, do not provide molecular structural information and cannot be used as a principal means of identification. Given the increasing emphasis on instrumental methods of analysis and the limitations of the traditional instruments in the field of forensics, another solution is necessary. This application note describes the method to develop a UHPLC/MS method for detection, separation and confirmation of psilocybin and psilocin in mushrooms and chocolate matrices.
Download Full Article [PDF 1.84 MB]
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In collaboration with Washington State Patrol - Crime Laboratory Division, Cheney, Washington, USA
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| Proteomics Seminar Series (Oct - Nov, 2008, Europe) |
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The Thermo Scientific Proteomics Seminar Series has become an established venue for scientific discussion and sharing of practical tips among the users of Thermo Scientific mass spectrometers. We hope that you will be able to join us for a FREE seminar at a location near to you!
This year's theme is protein quantification and the seminars will provide you with the opportunity to get first-hand information about our relative and absolute quantification workflows in proteomics:
- Biomarker candidate discovery and verification
- Labeling techniques (SILAC, TMT and iTRAQ)
- Label-free approaches
- Absolute quantification of proteins by multiple SRM
Learn More and Register
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| Clinical Research: Quantitating Vitamin D using TurboFlow Technology |
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Quantitation of Vitamin D is a commonly used method in clinical research. This application note will show you a rapid (5 minute), sensitive (LOQ 4 ng/nL or 10 nMol/L) and reliable LC/MS method for analysis of 25-hydroxy-vitamin D3 and 25-hydroxy-vitamin D2 in serum. Learn more about how TurboFlow™ technology and LC/MS fit in a clinical research setting.
Download Full Article [PDF 338 kB]
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Food Safety: Determination of Pesticide Residues in Processed Fruits and Vegetables According to EU Directive 91/414 EEC
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By researchers at General Chemical State Laboratory, Pesticide Residues Laboratory, Athens, Greece
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A diet rich in fruits and vegetables is thought to reduce the risk of some types of cancer, atherosclerosis, and heart disease. However, commercially grown produce often contains high levels of pesticide residues that can lead to serious health problems when consumed. Due in large part to growing public concern over the amount of pesticide residues in foods, the European Union (EU) has enacted several directives to fix Maximum Residue Limits (MRLs) for different pesticide residues in food of plant origin. In this application note a multi-residue LC-ESI-MS/MS method was developed for the reliable confirmation and quantification of pesticides from different chemical classes at low ppb levels in food matrices.
Download Full Article [PDF 862 kB]
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| Bioanalysis: Paroxetine in Rat Plasma - Enhanced Reproducibility Performance of the TSQ Vantage |
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In May 2001, the FDA promulgated its first official guidance for bioanalytical methods. This document is titled Guidance for Industry Bioanalytical Method Validation, and serves as a guide for bioanalytical methods supporting clinical and non-clinical studies. Recently, the reproducibility and accuracy of the concentration determined in incurred samples was subject to significant debate, outlined in the American Association of Pharmaceutical Scientists (AAPS) journal paper titled Workshop/Conference Report - Quantitative Bioanalytical Methods Validation and Implementation: Best Practices for Chromatographic and Ligand Binding Assays.
Download Full Article [PDF 274 kB]
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28th Montreux Symposium on LC/MS (Nov 12-14, 2008, Montreux, Switzerland)
Thermo Scientific User Meeting Nov 11, 2008 |
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Visit us at the 28th annual Montreux symposium to learn about the range of Thermo Scientific products, technologies and applications. Learn about two of the newest products that were designed to solve your problems:
- The Exactive is the new high-performance LC-MS system for high-throughput applications with confident compound identification
- The TSQ Vantage triple stage quadrupole mass spectrometer delivers the highest sensitivity with the lowest noise for the quantitative analysis of small molecules, peptides, biosimilars and biologics
Learn More and Register
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| Trap Talk: The Exactive Benchtop LC/MS Orbitrap Mass Spectrometer |
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Over the past three years the combination of Orbitrap technology combined with a linear ion trap has become an established platform for high resolution, accurate mass LC/MSn analysis. The high resolving power, mass accuracy and dynamic range of the Orbitrap analyzer allow rigorous characterization of complex mixtures even in the absence of precursor ion mass selection. This note describes the development of a non-hybrid mass spectrometer comprising of an atmospheric-pressure ion source (API) and a standalone Orbitrap mass analyzer.
Download Full Article [PDF 745 kB]
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