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Life Science Mass Spectrometry
| Tip of the Month: Live Webinar - High Throughput in the LC-MS/MS Laboratory - Enabling Technologies for Integrated Sample Workflows (Nov 19, 2008, 11 A.M. U.S. Eastern Time) |
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The traditional options for sample preparation include solid phase extraction, protein precipitation or liquid extraction. In the last 10 years, an on-line sample extraction technique using turbulent flow chromatography has provided a way to minimize many of the drawbacks of traditional methods. In this webcast the advantages and disadvantages of these methods will be compared to a more recent solution utilizing turbulent flow chromatography.
This is relevant to those who are doing analysis with biological fluids and having trouble with sample prep, users of high throughput LC-MS/MS, and scientists developing methods for the analysis of small molecules in biological fluids.
Key Learning Objectives:
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Primer of Thermo Scientific TurboFlow technology, columns and systems
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Simultaneous improvements to data quality and throughput
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Illustrations from a variety of real-world sample extraction challenges
Learn More and Register
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| Food Safety: Determination of Melamine and Cyanuric Acid Residues using LC-MS/MS |
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In March 2007, several North American manufacturers of pet food voluntarily issued nationwide recall notices for some of their products that were reportedly associated with renal failure in pets. The raw material wheat gluten, used to manufacture the pet food, was imported from China. Although initial reports suggested that contamination was confined to pet food, further investigations revealed that melamine tainted fodder may have been used to feed animals intended for human consumption.
In particular, it was discovered that melamine-contaminated ingredients had been used to prepare feed for chickens, swine, and catfish. Consequently, the U.S. Food and Drug Administration (FDA) and the U.S. Department of Agriculture (USDA) have developed methods for the analysis of melamine residues in animal tissue. Both methods use tandem mass spectrometric detection and employ disposable strong cation exchange solid phase extraction (SPE) cartridges to prepare samples for liquid chromatographic analysis.
Download Full Article [PDF 673 kB]
Read U.S. FDA Laboratory Information Bulletin [U.S. FDA link]
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28th Montreux Symposium on LC/MS (Nov 12-14, 2008, Montreux, Switzerland)
Thermo Scientific Users Meeting Nov 11, 2008 |
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Visit us at the 28th annual Montreux symposium to learn about the range of Thermo Scientific LC/MS products, technologies and applications. Sign up for our users meeting and lunchtime workshops to find out about two new LC/MS products:
- The Exactive™ high-performance LC-MS system is suited for high-throughput applications requiring confident compound identification
- The TSQ Vantage™ triple stage quadrupole mass spectrometer delivers the highest sensitivity with the lowest noise for the quantitative analysis of small molecules, peptides, biosimilars and biologics
Learn More and Register
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| Clinical Research: Quantitative Analysis of Cortisol and Cortisone in Urine by LC-MS/MS |
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A fast, sensitive and reliable LC-MS/MS SRM method has been developed for the determination of cortisol and cortisone in urine. Sample analysis was performed with a runtime of 10 minutes with a quantification limit of 0.12 µg/mL for cortisol and a linearity range of 0.12 - 20 µg/mL for cortisol. The quantification limit for cortisone is 0.20 - 20 µg/mL. The low intra-assay and inter-assay variability of the results demonstrates the reliability of the method.
Download Full Article [PDF 832 kB]
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Proteomics Seminar Series (Oct - Nov 2008, Europe)
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The Thermo Scientific Proteomics Seminar Series has become an established venue for scientific discussion and sharing of practical tips among the users of our mass spectrometers. We hope that you will be able to join us for a FREE seminar at a location near to you!
This year's theme is protein quantification and the seminars will provide you with the opportunity to get first-hand information about our relative and absolute quantification workflows in proteomics:
- Biomarker candidate discovery and verification
- Labeling techniques (SILAC, TMT™ and iTRAQ™)
- Label-free approaches
- Absolute quantification of proteins by multiple SRM
Learn More and Register
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| Proteomics: Quantitation of iTRAQ Labeled Peptides using Higher Energy Collisional Dissociation on the LTQ Orbitrap |
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Accurate relative quantitation of differentially expressed proteins and protein isoforms based on the corresponding complex peptide mixtures continues to be an analytical challenge. The iTRAQ technology based on low mass 'reporter' ion abundances in MS/MS scans provides a means of performing simultaneous peptide identification and relative quantitation for up to eight samples. LC-MS/MS using a linear ion trap is a well established strategy for highly sensitive and robust protein identification in bottom-up experiments, and can also be used for quantitation of the low mass iTRAQ reporter ions created using MS3 or Pulsed-Q Dissociation (PQD) methods. The primary goal of this work was to systematically optimize the experimental conditions for relative quantitation of iTRAQ labeled peptides using HCD on the LTQ Orbitrap XL™.
Download Full Article [PDF 735 kB]
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| Metabolism: Identification of GSH Conjugates using Accurate Mass Data and MetWorks Software |
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In collaboration with Heng-Keang Lim, Johnson and Johnson Pharmaceutical Research Institute, Raritan, New Jersey, USA
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Incorporating metabolite profiling for ADME properties early in discovery across the pharmaceutical industry has resulted in an increase in drug success in development by decreasing failure due to poor pharmacokinetic properties. However, since it is difficult to predict how toxicity screens compare to human toxicity in vivo, there has been a shift to drug attrition during development due to preclinical or clinical safety issues. This is due to the fact that there are no animal models that adequately predict drug toxicity involving the cardiovascular and hepatic systems in humans. In order to avoid drug toxicity in development, the screening for formation of electrophilic reactive intermediates has been widely embraced by the pharmaceutical industry as part of drug design. In this report, a cofactor-fortified human liver microsomal incubation of 10 µM of nefazodone was analyzed using the Thermo Scientific LTQ Orbitrap which also incorporated use of isotopic pattern triggered data-dependent high-resolution accurate mass analysis.
Download Full Article [PDF 519 kB]
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| Environmental: Analyzing Phenolic Pollutants in Water using U-HPLC |
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Phenolic compounds are of particular environmental importance due to their relatively high toxicity at low levels and their presence in environmental waters and organic matter, following degradation of a range of industrial products such as pesticides and herbicides, as well as naturally occurring humic substances and tannins. Previous studies have shown that reversed-phase liquid chromatography (RP-LC) coupled to atmospheric pressure chemical ionization mass spectrometry (APCI-MS) can effectively separate and detect a range of phenolic compounds at low ppb levels, following various extraction methods. In this study, the effect on the separation and analysis speed of a number of priority phenols cited within the U.S. Environmental Protection Agency (EPA) and European Union (EU) lists of priority pollutants has been assessed by changing the chemistry and reducing the particle size of the stationary phase.
Download Full Article [PDF 553 kB]
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| 6th Uppsala Conference on Electron Capture and Transfer Dissociation (Dec 7-10, 2008, Madison, Wisconsin) |
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The 6th Uppsala Conference on Electron Capture and Transfer Dissociation will be held in Madison, WI on December 7-10, 2008. The program offers an outstanding lineup of internationally-known speakers and will be held at the Frank Lloyd Wright designed Monona Terrace Conference center in downtown Madison. Registration is now open and discounted registration is available for student participants.
Learn More
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| Trap Talk: Study of free Radical Fragment Ions Generated from ESI-CID-MS/MS using Thermo Scientific LTQ and LTQ Orbitrap Mass Spectrometers |
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Collision induced dissociation (CID) has been extensively used for structure elucidation. CID in the ESI and APCI modes has been found to generate mostly even electron fragments while it has been occasionally reported to form odd-electron free radical ions. However, the structural requirement and the fragmentation mechanisms for free-radical CIDs have not been well characterized in the literature. For this purpose, studies of aromatic and nonaromatic compounds such as sulfonamides, amides, aromatic t-Bu compounds, aromatic ether, oxime ethers and pyrimidines were undertaken using the LTQ™ and LTQ Orbitrap™ mass spectrometers. The results showed that free radical fragmentation is structure dependent and is directly correlated with the functional groups in the structure that stabilize the newly formed free radicals.
Download Full Article [PDF 1.11 MB]
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