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Life Science Mass Spectrometry
| Tip of the Month: SRM Transitions Using the EZ-Method Function in Thermo Scientific TSQ Series Version 2.0.5 Software |
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When building a new TSQ™ Series method in Quantum version 2.0.5 or higher, you have the choice to use "EZ-Method" or "Regular Method." The choice can be found under the menu "TSQ." EZ-Method allows you to assign compound names to your SRM transitions, as well as define a start time and a stop time to scan for that SRM transition. You no longer have to assign time segments to groups of transitions, making method development for large numbers of analytes much easier!
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| Proteomics: Quantitative Profiling of DNA Damage Response Proteins Using iTRAQ™ Labeling and the Thermo Scientific LTQ Orbitrap XL |
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Quantitative proteome analysis facilitates the study of gene expression, and protein concentrations often change in response to DNA damage and other cellular stresses such as post-translational modification, namely, phosphorylation. This application note illustrates the methodology for reproducibility enriching for the phosphoproteins under study and using the HCD cell in the LTQ Orbitrap XL™ mass spectrometer to accurately and precisely quantify the iTRAQ-labeled peptides.
» Download Full Application Note [PDF 747 kB]
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| Proteomic Forum 2009 (March 29 - April 2, Berlin, Germany) |
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Visit the Thermo Scientific booth (booth 0.4 on the ground floor) at the Proteomic Forum 2009 in Berlin. Learn about the latest Thermo Scientific mass spectrometry based products and workflows applied to proteomics - including the LTQ Orbitrap series and the new Thermo Scientific TSQ Vantage triple quadrupole. Attend the technical presentations by our scientists and users and join us at our lunchtime workshop on Tuesday starting at 12:45 to hear from customers using our products to solve biological problems.
» Learn more and Register
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| Pittcon 2009 (March 8 - 13, Chicago, IL, USA) |
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Your Passport to Science -- Booth 1432
Visit the Thermo Scientific booth to see the latest innovations in mass spectrometry and a showcase of solutions that deliver scientific success and accelerate discovery. From analytical instruments and cutting-edge lab equipment to software solutions and expert services, let Thermo Scientific experts be your guides and learn how our integrated solutions can help solve your most challenging applications.
Pick up your official Passport to Science at the Thermo Scientific Information Desk in Booth 1432. It's your official map to the world of Thermo Scientific products and it's your ticket to receive a FREE gift
» Learn more and Register
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Bioanalysis: FAIMS Removes Interference in a Validated Small Molecule Bioanalytical Assay
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Representative LC-FAIMS-
MS/MS chromatograms
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Bioanalytical methods are developed to determine drug concentration and must be validated using the same matrix as the study subjects. The ideal control matrix contains no interferences, but this must be tested during the validation according to FDA guidelines. Occasionally, individual defined sources, or lots, of matrix show interferences. These matrices contain numerous components which may interfere with MS analysis. Other factors related to compound administration may also contain potential interferences for the bioanalytical assay.
To improve the selectivity of the assay, high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) was used. FAIMS increases the selectivity of analysis by selecting which ions are allowed into the MS. This technique filters out interferences and leaves the signal cleaner than LC-MS/MS alone.
» Download Full Application Note [PDF 467 kB]
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| Metabolism: Request a Complementary Biotransformation Reference Guide |
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This laminated guide lists in a tabled format accurate masses and mass defects of common Phase I and Phase II biotransformations. Use it as a quick reference guide in your laboratory.
» Request your guide now!
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| Clinical Research and Forensics: A Complete Toxicology Screening Procedure for Drugs and Toxic Compounds in Urine and Plasma Using LC-MS/MS |
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Step-by-step application workflow
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Toxicology laboratories commonly use automated immunoassays, gas chromatography-mass spectrometry (GC-MS) and high pressure liquid chromatography-diode array detector (HPLC-DAD) techniques to perform toxicology screening analyses. None of these techniques are able to identify all the drugs and toxic substances.
The benefits of the LC-MS/MS screening methodology include a simple sample preparation procedure, ease of adding new compounds to the screening method and fewer limitations based on compound volatility and thermal stability. In addition, Thermo Scientific ToxID automated toxicology screening software is able to automatically generate both Summary and Long Reports, avoiding the need for manual analysis of each sample chromatogram.
» Download Full Application Note [PDF 521 kB]
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| On-Demand Webinar: Understanding the Move to Fast LC |
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Small sub-2um particle packed HPLC columns provide many opportunities for reducing time and cost. Some of the advantages include speed, resolution and sensitivity. This webinar details these advantages and how to benefit from them.
» View this On-Demand Webinar
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| Chromatography: Minimizing the Impact of the Global Acetonitrile Shortage |
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The global shortage of acetonitrile is causing concern for many HPLC and LC/MS operators. This article presents a number of solutions to help reduce or replace acetonitrile in test methods.
» Learn more
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| Complex Matrices: Direct Analysis of Red Wine Using Ultra-Fast Chromatography and High Resolution Mass Spectrometry |
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Red wine is a very complex mixture and a rich source of beneficial anti-oxidants. Identification and quantitation of these natural products is challenging. Ultra High Pressure Liquid Chromatography (U-HPLC) coupled to the LTQ Orbitrap XL mass spectrometer was used for analysis of French red wine, which enabled simultaneous detection and relative quantitation of the wine's anti-oxidant constituents.
» Download Full Application Note [PDF 502 kB]
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| Trap Talk: Profiling and Characterization of Polyphenol Polymers from Cinnamon Using an Ion Trap Mass Spectrometer |
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Type 2 diabetes is the most common form of the disease. Although the prevention and control of it remains unclear, there is strong evidence that dietary factors play a role in its onset. Previous studies have indicated that cinnamon may mimic insulin effects and thus may improve glucose uptake. Considering the complex composition of cinnamon, it is important to be able to isolate and characterize its possible active components. A range of reports have been devoted to the studies of this topic. However, most work has been focused on the studies of monomer and dimers. The study of trimer or even larger compounds using an on-line LC/MSn approach has not been reported.
In this application note, profiling and structural elucidation of polyphenol polymers, the possible active compounds in cinnamon, are carried out using an LC/MSn methodology using the Thermo Scientific LCQ Fleet ion trap mass spectrometer.
» Download Full Application Note [PDF 828 kB]
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