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Life Sciences Mass Spectrometry
| Tip of the Month: Thermo Scientific Proteome Discoverer 1.0 Operations |
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Using Filters and Peptide Confidence Together
In Proteome Discoverer software there are two processes that can be used together: "Peptide Confidence" and "Filters". The "Peptide Confidence" is measured using the Decoy Database searching built into Proteome Discoverer (PD). PD will compute what scores are needed for each peptide to have a Low, Medium, and High confidence. These designations are shown in the peptide color in the 'Proteins' display. If the user does not want to display the lower confidence hits, they can use the 'Filters' tab to remove them from the "Proteins" display. See Figure 1 [PDF 185 kB].
» More information [www.thermo.com/education]
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Accelerate Your Discoveries With the New LTQ Velos and LTQ Orbitrap Velos Mass Spectrometers
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Introducing the world’s fastest and most sensitive ion trap-based technologies:
- The Thermo Scientific LTQ Velos features a new dual-pressure trap design and API source, making it the world's fastest and most sensitive ion trap mass spectrometer. The unique dual-pressure trap technology enables the utilization of optimal pressures during analysis, increasing both scan speed and resolution.
- The Thermo Scientific LTQ Orbitrap Velos mass spectrometer delivers unsurpassed, ultra-high resolution and mass accuracy at unprecedented speeds for absolute confidence in compound identification.
Find out how these two new innovations can enhance your analytical capabilities.
» Learn more [www.thermo.com/velos]
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On-demand Webinar:
Introducing the Next Generation of Ion Trap and Orbitrap Mass Spectrometers: Two New LC/MS Systems for Confident Protein ID and Structural Elucidation
The LTQ Velos and LTQ Orbitrap Velos mass spectrometers feature new technologies that rapidly deliver the most confident protein and metabolite identification, characterization and quantitation. Find out how unique dual-pressure trap technology and API source improvements enable the identification of more low abundance compounds in less time. Access this on-demand webinar to learn how to maximize these technologies for the analysis of complex mixtures - increasing protein coverage and gaining access to exceptional MSn structural elucidation data.
» Register Now!
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| Drug Discovery: Precursor Ion Fingerprinting - Searching for a Structurally Diagnostic Fragment Using Combined Targeted and Data Dependent MSn |
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There is an increasing desire to reduce development time and cost devoted to ill-fated lead candidates and therefore a growing need for complete characterization of each compound earlier in the discovery process. Discovery DMPK experiments are becoming routine, resulting in a need for more rapid and automated methods of verification that a putative metabolite is related to the parent drug. This application note presents a simplified Precursor Identifying Fragment (PIF) technique which readily lends itself to routine automation and that offers the advantage of metabolite identification with no a priori knowledge of the active pharmaceutical ingredient (API) or any biotransformation products.
» Download Full Application Note [PDF 634 kB]
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| Clinical Research: Quantitation of Fentanyl and Norfentanyl from Urine Using On-line High Throughput System |
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Increasing demand in clinical research laboratories for higher sample throughput has put the emphasis on automated methods and platforms that have the ability to quickly ramp up throughput to meet demand. This application note demonstrates how to run 1 sample every 86 seconds with a 92.9% sample completion rate with 7.1% re-injection. The method run time was 5.5 minutes.
» Register to Download Full Application Note [PDF 179 kB]
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Bioanalysis / Proteomics: Linear Dynamic Range in Small Molecule & Targeted Peptide Quantitation
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The benefit of wide linear dynamic range is important for small molecule quantitation during drug research, and for targeted peptide quantitation. During drug research, the dosage form can cause an order of magnitude difference in quantitative results. In proteomics, the dynamic range of proteins in real samples can be in excess of 10 orders of magnitude with further complications arising from varying ionization efficiencies for the representative enzymatic peptide fragments. Thus having a wide dynamic range makes experimentation simpler by avoiding re-assays and dilution issues.
» Download Full Application Note [PDF 284 kB]
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| Chromatography: HPLC Method Development Calculator |
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Many chromatographers are considering moving their current HPLC methods to new columns geometries and particle sizes to improve speed, resolution and/or sensitivity. To help simplify this process Thermo Fisher Scientific has developed method transfer calculators for isocratic method and gradient method transfer. Just simply enter your current conditions and the new conditions under consideration to see what effects can be achieved.
» Gradient Method Transfer Calculator
» Isocratic Method Transfer Calculator
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Register for our Annual ASMS Users' Meeting and Customer Event
(May 31, 2009, Philadelphia, PA, USA) |
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Join us Sunday May 31st from 8 AM - 12 PM in Philadelphia, PA to find out about new methods and instrumentation for resolving complexity in your mass spectrometry analyses. The annual users' meeting will feature a plenary presentation from Jesper Olsen, Ph.D, from the Max Planck Institute for Biochemistry, followed by break-out sessions on small molecule and large molecule applications. After an informative morning, we'll transport you to the Moshulu, the largest four-masted sailing ship in the world still afloat, for lunch with unparalleled views of the city and waterfront.
» View Agenda
» Register Now!
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| Upcoming European Events |
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Moving Science Forward
Visit the Thermo Scientific booths for more information about products and workflows to meet your challenges.
For more information:
» SETAC Europe 19th Annual Meeting, May 31 - June 4, 2009, Goteborg, Sweden
» 3rd European Proteomics Association (EuPA), June 14 - 17, 2009, Stockholm, Sweden
» Goldschmidt 2009, June 21 - 26, 2009, Davos, Switzerland
» 7th International Conference on Homocysteine Metabolism, June 21 -2 5, 2009, Prague, Czech Republic
» MediLS Summer School on Computational Mass Spectrometry-Based Proteomics, June 27 - July 2, 2009 in Split, Croatia
» HPLC 2009, June 28 - July 2, 2009, Dresden, Germany
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| Trap Talk: Dual-Pressure Linear Ion Trap Technology |
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The LTQ Velos™ mass spectrometer incorporates a revolutionary dual pressure linear ion trap design. Prior to the LTQ Velos dual-pressure design, ion traps were operated at a single pressure that was a compromise between the optimum pressures for ion manipulation (trapping, isolating, fragmenting) and detection (mass analysis). The unique dual-pressure linear ion trap technology features two discrete pressure regions (cells) that allow the decoupling of ion manipulation and detection. A high-pressure cell (HPC) is used for trapping injected ions, isolating precursor ions, and fragmenting precursor ions. A low-pressure cell (LPC) is used for scanning ions out to the detectors, i.e. mass analysis.
» Download Full Product Support Bulletin [PDF 230 kB]
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