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Life Sciences Mass Spectrometry
| Tip of the Month: Improving ETD Activation Times |
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For increased coverage of complex sample mixtures, the activation time used for electron transfer dissociation (ETD) fragmentation of higher charge state peptides should be around 50ms. However, upon tuning the ETD activation time for +3 and higher charge states, you may find that the optimized activation time is significantly greater than this recommended value. An easy way to reduce the activation time is to increase the reagent ion automatic gain control (AGC) target setting from its default value of 1.00e+05.
» Download 5-step instructions to see how! [PDF 123 kB]
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Thermo Scientific Course Promotions: Special Offer - 25% Off on Select Courses*
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Special Offer
Bring a friend when you enroll in a Thermo Scientific US training course at regular price, and your colleague will receive 25% off the standard price. That’s huge savings for your organization and a familiar face in the classroom.
Upcoming courses include:
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TSQ Quantum/TSQ Vantage Operations, July 20-23, West Palm Beach, FL.
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LTQ Orbitrap XL Operations, July 27-30, West Palm Beach, FL.
» See the full list of discounted courses!
*OFFER VALID IN THE UNITED STATES ONLY
For more information:
» North America Training Institute - LIFECYCLE Education Services
» European Training Institute
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| Bioanalysis: Using FAIMS to Remove Interference in a Validated Small Molecule Assay |
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Representative LC-MS/MS
chromatograms for the analyte at the LLOQ
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Bioanalytical methods are developed to determine drug concentrations and must be validated using the same matrix as the study subjects. The ideal control matrix contains no interferences, but this must be tested during the validation according to FDA guidelines. High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) increases the selectivity of analysis by selecting which ions are allowed into the mass spectrometer. This technique filters out interferences and leaves the signal cleaner than LC-MS/MS alone.
» Download Full Application Note [PDF 472 kB]
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| Forensic Analysis: Analyzing Opiates in Whole Blood with Online Sample Extraction and LC-MS/MS |
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The opiate morphine, and its derivatives, are medicines often used for pain-relief, cough-relief and as anti-diarrhoeals. For example, codeine and dihydrocodeine (morphine derivatives) are available in over-the-counter preparations in combination with paracetamol (acetaminophen) and are slowly metabolized to morphine and dihydromorphine respectively. The forensic toxicologist is often asked to interpret results and possibly account for time of death in opiate (especially heroin) abuse cases. This task can be made easier if it is possible to identify and quantify the components such as 6-MAM, morphine, codeine, dihydrocodeine and the glucuronides in whole blood rather than urine.
» Register to Download Full Application Note
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Environmental: Case Study - Online Sample Extraction and LC-MS/MS for Water Analysis
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The Environmental Analytical Chemistry Laboratory at the University of Montreal comprises approximately 20 researchers focused on finding the best analytical methods for environmental studies. The laboratory is currently collaborating with the City of Montreal, the Drinking Water Research Chair at the École Polytechnique de Montréal, and Environment Canada in order to identify and quantify pharmaceutical residues in the city's waste and drinking water. To conduct this research, the University has developed a new online sample preparation method using a customized Thermo Scientific EQuan system for solid phase extraction coupled to liquid chromatography and tandem mass spectrometry (SPE-LC-MS/MS) analysis.
» Register to Download Full Case Study
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| On-Demand Webinar: High Throughput in the LC-MS/MS Laboratory - Enabling Technologies for Integrated Sampling Workflows |
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Increase Your Mass Spec Efficiency!
This free on-demand webinar compares traditional sample preparation techniques - SPE, LLE, PPT - with a new, online sample extraction technique. Learn how to reduce sample prep up to 95% and increase sample throughput up to 4X.
» Register to lean more!
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| Chromatography: Fast Analysis of Water-Soluble Vitamins |
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Water-soluble vitamins represent a group of various, essential dietary compounds, which are classified by their biochemical activities, not their physico-chemical properties. A single vitamin generally is formed by several vitamers (various chemical species, each of which displays the same biological activity). As a result of their different chemical properties, it can be difficult to reliably and simultaneously analyze vitamins. This application note describes a fast LC method for the analysis of water-soluble vitamins using a Thermo Scientific Hypersil GOLD aQ column.
» Download Full Application Note [PDF 139 kB]
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| Upcoming Events |
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Resolving Complexity
Visit the Thermo Scientific booths for more information about products and workflows to meet your challenges.
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Europe
» 6th PMF Meeting, July 1-2, 2009, University of Leicester, UK [Proteomics]
» Metabomeeting 2009, July 5-8, 2009, Norwich, UK [Metabolomics / Metabonomics]
» BSPR-EBI Proteomics Meeting, July 14-16, 2009, Cambridge, UK [Proteomics]
» 11th International Congress on Amino Acids, Peptides and Proteins, August 3-7, 2009, Vienna, Austria [Proteomics]
» TIAFT 2009, August 23-27, 2009, Geneva, Switzerland [Forensic Toxicology]
» 18th IMSC 2009, August 30 - September 4, 2009, Bremen, Germany [Mass Spectrometry]
North America
» Florida Pesticide Residue Workshop, July 19-22, 2009, St. Beach, FL, USA [Environmental and Food Safety]
» American Association for Clinical Chemistry (AACC), July 19-23, 2009, Chicago, IL, USA [Clinical]
» CoSMoS 2009, August 3-5, 2009, Boston, MA, USA [Small Molecule]
» Mass Spec 2009, September 15-18, 2009, Philadelphia, PA, USA [Mass Spectrometry]
» HUPO 8th Annual World Congress, September 26-30, 2009, Toronto, Canada [Proteomics]
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| Proteomics: Analysis of Glycopeptides Using Porous Graphite Chromatography and LTQ Orbitrap XL ETD Hybrid MS |
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Glycosylation of Asn, Ser or Thr is arguably the most common known post-translational modification (PTM), resulting in a multitude of highly heterogeneous protein isoforms. Traditional electrophoretic and CID LC-MS/MS approaches to analysis of glycopeptides have significant limitations. This application note demonstrates the advantages of a workflow that incorporates porous graphite chromatography and electron transfer dissociation (ETD) LC-MS/MS for identification and characterization of glycopeptides.
» Download Full Application Note [PDF 747 kB]
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| Trap Talk: Novel Dual-Pressure Linear Ion Trap Mass Spectrometer Offers Breakthrough Performance in Proteomics Experiments |
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This application note addresses the challenging analysis of a complex, multi-organ peptide digest of Caenorhabditis elegans (C. elegans) to assess the performance of the innovative new Thermo Scientific LTQ Velos mass spectrometer. Performance of this new instrument is benchmarked against the previous state-of-the-art linear ion trap and also against a well-known quadrupole time-of-flight mass spectrometer.
» Download Full Application Note [PDF 693 kB]
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