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Life Sciences Mass Spectrometry
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Tip of the Month: Quantitative and Qualitative Data... All at Once Using the QED Scan Function
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In some analytical arenas, especially when dealing with complex matrices, having one quantitative selected reaction monitoring (SRM) scan event is not enough for positive confirmation of a species when doing low-level quantitation. A popular method of confirmation for the analyte of interest is to perform multiple scans looking for different product ions (typically called MRM or multiple reaction monitoring) and then confirming the additional ions are present in a appropriate ration (ion ration confirmation or IRC). A secondary method, available on all Thermo Scientific TSQ Quantum instruments, is to perform a QED scan (Quantitation Enhanced Data Dependent). The QED scan is a second scan event performed after the first SRM scan event that is a total product ion scan. Thus, instead of looking at one or two additional product ions, all product ions can now be used for confirmation of the analyte of interest.
» Learn More About the QED Scan Function by Viewing this Webinar [19 minutes]
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Proteomics: In-Depth, Comprehensive Mapping of the Human Seminal Plasma Proteome by a Novel, Iterative LC-MS/MS Analysis and Database Search Workflow
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Seminal plasma is a highly complex fluid that contains proteins secreted from several glands of the male reproductive tract: prostrate, seminal vesicle, epididymis, and testis. However, like most body fluids, seminal plasma presents a wide range of protein concentrations that renders low-abundance proteins very difficult to identify and quantify.
This application note describes a method for extensive protein identification in complex samples that involves iterative nanoflow LC-MS/MS analysis, exclusion list generation, and iterative database searching with Thermo Scientific Proteome Discoverer software, leading to the identification of numerous low-copy-number proteins in the seminal plasma proteome.
» Download Complete Application Note [PDF 1.73 MB]
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Proteomics: On-Demand Webinar
Fast and Effective Digested Protein Complex Sample Clean-up for LC/MS/MS
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Protein Purification Webinar by Dr. Catherine Wong
The Scripps Institute
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Salts, detergents, and other contaminants can suppress ionization and negatively impact LC-MS and LC-MS/MS analysis of proteins. In this recorded webinar, Dr. Catherine Wong from The Scripps Research Institute discusses how she uses Thermo Scientific Aspire RP30 chromatography pipette tips with a proprietary reversed-phase resin as part of a 20-minute purification protocol to recover peptides from complex protein digests. The result is an increase in signal-to-noise ratio, sequence coverage, and peptide/protein identification compared to sample preparation with conventional C18 products.
» Register to View the On-Demand Webinar [21 minutes]
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Proteomics Seminar Series: An Insight into Quantitative Proteomics
(September - November 2009, USA)
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A series of 22 free half-day seminars will be held at locations across North America September 9th – November 5th. Academic, industry, and Thermo Fisher Scientific representatives will discuss recent innovations in, and approaches to, qualitative and quantitative proteomics. Registration is limited so please sign up now.
» Visit the Seminar Website to Find a Seminar in Your Area and Register
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Food Safety & Environmental: LC-MS/MS Analysis of Herbicides in Drinking Water at Femtogram Levels
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As concerns grow over the toxic effects of herbicides and other chemicals in our environment, the need to accurately monitor these substances in drinking water and foods becomes even more critical. LC-MS/MS is routinely used by the environmental and food industries to identify and quantify pesticide and herbicide residues. However, this method typically requires extensive offline sample pre-concentration methods, which can be expensive and time-consuming, to meet the stringent requirements and low limits of detection set forth by federal and international regulatory authorities. An online pre-concentration and cleanup method has been developed that improves both sensitivity and precision and yields unmatched throughput.
This application note demonstrates how the large injection volume capabilities of the Thermo Scientific EQuan system eliminated the need for laborious and expensive offline pre-concentration using solid phase extraction.
» Download Complete Application Note [PDF 345 kB]
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| Bioanalysis: Increasing Throughput on GLP-Level Quantitative Analysis |
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The massive quantity of samples to be analyzed by LC/MS/MS in the pharmaceutical marketplace has driven the search for methods to increase throughput without sacrificing quality. The Thermo Scientific Aria LX-4 multiplexed HPLC system, combined with LC/MS/MS, significantly decreases the time required for applications of drug discovery and development without sacrificing selectivity or sensitivity.
This application note describes an efficient means of increasing throughput without the need for significant changes in current laboratory analytical protocols.
» Register to Download Complete Application Note [PDF 130 kB]
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| Chromatography: UHPLC Separation of Triazine Herbicides at Elevated Temperature |
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Temperature is a key variable in high performance liquid chromatography (HPLC), influencing solute diffusion rates, mobile phase viscosity, and solubility. For example, as column temperature increases, analyte diffusion increases. Increased analyte diffusion generally leads to an increase in the optimum linear velocity of the separation, so that the equivalent chromatographic efficiency and resolution can be achieved at a higher flow rate. Furthermore, elevating the temperature reduces the operating back-pressure. The net result is that separations can be performed faster without exceeding the pressure limitations of the instrument.
This application note demonstrates the performance of the high temperature liquid chromatographic method, including precision of retention time and peak area, resolution, and spike recovery from several environmental water matrices.
» Download Complete Application Note [PDF 577 kB]
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Upcoming Events
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Resolving Complexity
Visit the Thermo Scientific booths at these events for more information about products and workflows to meet your challenges.
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Europe
» 2009 Thermo Scientific Food Safety Seminars
September - December 2009, Europe [Food Safety]
» MipTec 2009
October 13-15, 2009, Basel, Switzerland [Drug Discovery]
» RAFA 2009, 4th International Symposium on Recent Advances in Food Analysis
November 4-6, 2009, Prague, Czech Republic [Food Safety]
» The European Bioanalysis Forum (EBF)
December 2-4, 2009, Barcelona, Spain [Bioanalysis]
» More Information- Upcoming Thermo Scientific Worldwide Events
North America
» 2009 Thermo Scientific Mass Spectrometry Users Meetings
October 6, 2009, Boston, MA, USA [Mass Spectrometry]
October 8, 2009, Somerset, NJ, USA [Mass Spectrometry]
» 15thNorth American ISSX Meeting
October 18-22, 2009, Baltimore, MD, USA [Metabolism]
» SOFT 2009, Society of Forensic Toxicologists Annual Meeting
October 18-23, 2009, Oklahoma City, OK, USA [Forensic Toxicology]
» 2009 Thermo Scientific Food Safety Seminars
October - November 2009, North America [Food Safety]
» EAS 2009, Eastern Analytical Symposium
November 16-19, 2009, Somerset, NJ, USA [Analytical]
» More Information- Upcoming Thermo Scientific North America Events
Asia Pacific
» 2009 Thermo Scientific Food Safety Seminars
October 5-16, 2009, Asia Pacific [Food Safety]
» More Information- Upcoming Thermo Scientific Worldwide Events
Japan
» 2009 Thermo Scientific Food Safety Seminars
October 21, 2009, Osaka, Japan [Food Safety]
October 23, 2009, Tokyo, Japan [Food Safety]
» More Information- Upcoming Thermo Scientific Worldwide Events
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Trap Talk: On-Demand Webinar
Introducing the Next Generation of Ion Trap and Orbitrap Mass Spectrometers |
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Two New LC/MS Systems for Confident Protein ID and Structural Elucidation
The Thermo Scientific LTQ Velos and LTQ Orbitrap Velos mass spectrometers feature new technologies that rapidly deliver the most confident protein and metabolite identification, characterization and quantitation. This webinar discusses how unique dual-pressure trap technology and API source improvements enable the identification of more low abundance compounds in less time. Access this on-demand webinar to learn how to maximize these technologies for the analysis of complex mixtures - increasing protein coverage and gaining access to exceptional MSn structural elucidation data.
» Register to View the On-Demand Webinar [1 hour]
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