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Electron Transfer Dissociation (ETD) technology

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ETD provides sequence information not available from conventional methods used in ion trap mass spectrometers

ETD enables peptide dissociation by transferring electrons to positively charged peptides, leading to a rich ladder of sequence ions derived from cleavage at the amide groups along the peptide backbone. Amino acid side chains and important modifications such as phosphorylation are preserved intact and can be readily assigned as a result of analysis.

The ETD source exploits the open architecture of the LTQ and is physically coupled to the back-end of the two-dimensional ion trap. The electrospray source remains on the front-end of the system.  This flexible design allows for the future addition of other ion reaction types (such as PTR).

Since large peptides can be successfully fragmented by ETD, this upgrade enables top-down and middle-down sequencing of small proteins/large peptides and supports bottom-up proteomics using enzymes that make larger peptide fragments.

Using ETD technology, your research will benefit from:

  • Enhanced protein identification and sequence coverage using bottom-up approaches
  • Improved identification of the location of post-translational modifications
  • Enhanced MS/MS of basic peptides and proteins such as histones
  • Much improved sequencing by MS/MS of large peptides and proteins
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Electron Transfer Dissociation (ETD) technology is now available for the award-winning Thermo Scientific LTQ Orbitrap XL hybrid mass spectrometer

The new LTQ Orbitrap XL ETD is the only ETD instrument with both high resolution and accurate mass, enabling definitive protein characterization. ETD technology enables highly sensitive post-translational modification analyses.

When used with the LTQ Orbitrap XL, ETD provides the most advanced proteomics platform available by offering three complementary fragmentation techniques in one instrument. These fragmentation techniques, CID, HCD and ETD, provide protein/peptide characterization; post-translational modification (PTM) analysis (especially phosphorylation); and top-down or middle-down sequencing of proteins and peptides.

The ETD system is currently available as an upgrade on Thermo Scientific LTQ and LTQ XL linear ion traps and LTQ Orbitrap XL hybrid mass spectrometry systems.

Learn more about proteomics and ETD options and technique in the following resources: